We determined the surface-associated proteolytic activity in three Entamoeba histolytica Schaudinn, 1903 strains (monoxenic HM1, axenic HM1, and HK9) of known virulence and its relationship with collagenase activity. Both activities were also determined in axenic HM1 amoebae trophozoites which were sensitive and resistant to complement-mediated lysis. Surface proteolytic activity was determined...

Digestive enzyme activity is often used as a sensitive response to environmental pollution. However, only little is known about the negative effects of stress on digestive capacities and their consequences on energy reserves and reproduction, although these parameters are important for the maintenance of populations. To highlight if changes in biochemical responses (digestive enzymes and reserv...

To understand the possible proteolytic contribution of yeast during cheese ripening, Debaryomyces hansenii 212 was isolated from commercial blue-veined cheese and incubated in a medium containing casein. Growth and casein degradation were recognized at the cheese-ripening temperature. Proteolytic activity was found in the intracellular fraction, and the enzyme, which was attached to the cell wa...

SOLUBILIZED PROTEIN DERIVED FROM HUMAN PLATELETS WAS FRACTIONATED BY DEAE CELLULOSE COLUMN CHROMATOGRAPHY AND ANALYZED FOR PROTEASE ACTIVITY USING THREE SUBSTRATES: denatured bovine hemoglobin, alpha casein, and purified plasminogen-free human fibrinogen. A protein fraction was found with proteolytic activity which was heat labile and not attributable to plasmin. The activity was not potentiate...

Plasminogen activator activity has been localized in gastric and duodenal tissue using a histological technique. This activity could be separated from proteolytic activity by incorporating in-aminocaproic acid into the fibrin substrate or by heat treatment. Plasminogen activator activity was found in relation to mucosal and submucosal blood vessels in the tissues studied. Proteolytic activity w...

introduction.?Snake venom is a secretion from the specialized glands in the mouth of the snake, analogous to the salivary glands of other animals. The venom itself is a complicated Protein and behaves like an enzyme. It is^ a neurotropic poison which is also endowed with digestive functions. Its strong proteolytic Properties are well known. A rat after being swallowed whole by a snake is comple...

Mammalian metallocarboxypeptidases play key roles in major biological processes, such as digestive-protein degradation and specific proteolytic processing. A Sulfolobus solfataricus gene (cpsA) encoding a recently described zinc carboxypeptidase with an unusually broad substrate specificity was cloned, sequenced, and expressed in Escherichia coli. Despite the lack of overall sequence homology w...

The biological testing of proteins before feed formulation, although desirable in terms of economy of protein useage; is often precluded by the time and money required to mount such assays, so it is not surprising that much work has gone into developing laboratory methods (Raynor and Fox 1976; Saunders and Kohler, 1972). An important determinant of quality is digestibility and many methods have...

Temperature and substrate availability constrain the activity of the extracellular enzymes that decompose and release nutrients from soil organic matter (SOM). Proteolytic enzymes are the primary class of enzymes involved in the depolymerization of nitrogen (N) from proteinaceous components of SOM, and their activity affects the rate of N cycling in forest soils. The objectives of this study we...

Proteases of Lactobacillus and Lactococcus strains catalyze casein degradation in fermented dairy products, which can result the production bioactive peptides. Proteolytic properties a selection lactic acid bacteria (LAB) previously isolated Croatia, including lactis strains, are described. All showed an Fmc+ phenotype that be associated with efficient growth milk. The significant caseinolytic ...