These are a water-bath at 37°C., with a rack to contain 10 x 70 mm. test tubes, two pipettes, 0 05 ml. for the blood sample and 0-25 ml. for the reagent,1 a sterile lancet, and a stop watch. six subjects with the adsorbed plasma described above. The results are plotted on double logarithm graph paper. Alternatively, a prothrombin ratio can be employed which obviates the necessity for the diluti...

Quick and Stefanini in 1948 introduced a method for estimating the activity of factor V (Owren, 1947a) in human and animal oxalated plasma. This factor is also known as the labile factor (Quick, 1947) and prothrombin A (Quick, 1943). Stefanini's modification of this method is fully described in a later paper (Stefanini, 1951). The essential steps in this modified method are that factor-V-defici...

background: leishmaniases are a group of diseases caused by leishmania parasites .  growing of drug unresponsiveness in leishmaniasis patients necessitates the development of new drugs and accor­dingly a suitable assay is needed for evaluation of any modalities.  the aim of this study was to compare four drug assays methods, agar dilution, broth dilution, cylinder plate and disk diffusion, for ...

introduction: gene expression and purification of luciferases from the firefly, lampyris turkestanicus, and optimization of cellular atp measurements were performed. methods: cdna encoding luciferases from lampyris turkestanicus was transferred from pqe30 vector into pet28a expression vector and pltu28 was built. newly constructed vector was expressed in e. coli xl1 blue and the recombinant luc...