We report population-based concentrations, stratified by age, sex, and racial/ethnic groups, of dialkyl phosphate (DAP) metabolites of multiple organophosphorus pesticides. We measured dimethylphosphate (DMP), dimethylthiophosphate (DMTP), dimethyldithiophosphate (DMDTP), diethylphosphate (DEP), diethylthiophosphate (DETP), and diethyldithiophosphate (DEDTP) concentrations in 1,949 urine sample...

We have reported in a previous paper that dimethyl sulfoxide (DMSO) induces the formation of huge bundles of actin filaments in the nuclei of Dictyostelium mucoroides. The present study was performed to provide electron microscope data on the induction of nuclear actin bundles, illustrating both their formation and their reversion, as well as on the effects of various factors on the induction. ...

Ion-pair reverse-phase HPLC–inductively coupled plasma (ICP) MS was employed to determine arsenite [As(III)], dimethyl arsenic acid (DMA), monomethyl arsenic (MMA) and arsenate [As(V)] in Chinese brake fern (Pteris vittata L.). The separation was performed on a reverse-phase C18 column (Haisil 100) by using a mobile phase containing 10 mM hexadecyltrimethyl ammonium bromide (CTAB) as ion-pairin...

Various mutant strains were used to examine the regulation and metabolic control of the Calvin-Benson-Bassham (CBB) reductive pentose phosphate pathway in Rhodobacter capsulatus. Previously, a ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO)-deficient strain (strain SBI/II) was found to show enhanced levels of cbb(I) and cbb(II) promoter activities during photoheterotrophic growth in t...

Two monopodal (2,4-dichloro-6-(3-hydroxytyramine)-1,3,5-triazine) and tripodal (2,4,6-(3-hydroxytyramine)-1,3,5-triazine) s-triazine derivatives were prepared through the reaction of cyanuric chloride (2,4,6-trichloro-1,3,5-triazine) and 3-hydroxytyramine hydrochloride (dopamine hydrochloride). The structures of the compounds were identified by FT-IR, 1H NMR, 13C NMR, thermal analysis and eleme...

We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The glyoxalated nucleic acids are then subjected to electrophoresis through either acrylamide or agarose gels in a 10 mM sodium phosphate buffer at pH 7.0...

' Aided by Research Grant CA-11811 from the NIH and by research funds from the United States Veterans Administration. 2 The abbreviations used are: c/s-Ptll, cw-dichlorodiammineplatinum(H) (NSC 119875); c/s-PPC, m-dichloro(dipyridine)platinum (II); EATC, Ehrlich ascites tumor cells; TCA, trichloroacetic acid; HN2, nitrogen mustard; DMSO, dimethyl sulfoxide; MEM, Eagle's minimum essential medium...

We have examined the stacking interactions of N(2)-dimethyl-guanosine with the nucleosides, e.g., adenosine and cytidine, found adjacent to it in certain tRNA's, by the use of model compounds in which the trimethylene bridge was substituted for the ribose-phosphate-ribose linkage. From the hypochromism exhibited by synthetic 9-[3-(aden-9-yl)propyl]-2-dimethylaminopurine-6-one (IV) and by 9-[3-(...

MATERIALS Reagents AmpliTaq polymerase (Perkin Elmer) Bovine serum albumin (BSA) (nuclease-free; Boehringer Mannheim; stock 1.6 mg/ml) Dimethyl sulfoxide (DMSO) (Aldrich) DNA in ES PCR Lysis buffer (include positive and negative controls) dNTPs (Pharmacia; stock of the mix 10 mM) ES colonies in G418 selection (see Electroporating DNA into Embryonic Stem (ES) Cells and Selection) Light mineral o...

Amidination of aldolase, glyceraldehyde-3-phosphate dehydrogenase, tryptophan synthetase B protein, L-arabinose isomerase, and the catalytic subunit of E. coli aspartate transcarbamylase with the bifunctional reagent dimethyl suberimidate produces cross-linked proteins, with reaction predominating within oligomers. Disc electrophoresis of a modified protein on polyacrylamide gel in the presence...