A simple, rapid, and sensitive HPLC method was developed and validated to quantify Amphotericin B (AmB) in human plasma. AmB was extracted from spiked plasma by simple protein precipitation with methanol. The separation was performed on an XBridgeTM C18 (150 × 4.6 mm, 3.5 μm) column, with a mobile phase of acetic acid (0.73%) – acetonitrile (60:40, v/v) and at a flow rate of 1 mL/min. The elute...

Wastewater samples collected from two sewage treatment plants (STPs) in Guangzhou, China were acidified, solid-phase extracted (SPE) with Oasis HLB cartridges, followed by instrumental measurement by high performance liquid chromatography (HPLC) coupled with a diode array UV detector (DAD) and a fluorescence detector (FLD) for the occurrence and fate of antimicrobial compounds sulfadiazine (SDZ...

A new type of radiation detector, a p-type modified electrode germanium diode, is presented. It is shown that the prototype displays, for the first time, a combination of features (mass, energy threshold and background expectation) required for a measurement of coherent neutrino–nucleus scattering in a nuclear reactor experiment. First results are presented from its calibration using sub-kiloel...

Capillary electrophoretic profiling of hair fractions obtained by 0.25 M HCl treatment of the tissue with subsequent extraction of the solubilized fraction with chloroform-isopropanol (9:1, v/v) revealed clear differences between hair obtained from alopecia areata affected laboratory rats (both in hair obtained from non-affected areas and hair growing on once hairless patches) and controls. Dif...

A simple and accurate high-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) and evaporative light scattering detector (ELSD) was established for the determination of six bioactive compounds in Zhenqi Fuzheng preparation (ZFP). The monitoring wavelengths were 254, 275 and 328 nm. Under the optimum conditions, good separation was achieved, and the assay was fully v...

1 Quantum Interference & Entanglement Description (QIE) 2 Quantum Interference & Entanglement Pictures 3 Before the 1st Day of Lab Objectives for this experiment 4 Introduction 4.1 Bell's Theorem and the CHSH Inequality 5 Experimental Setup 5.1 Overview 5.2 Laser Diode 5.3 BBOs 5.4 Detection 5.5 Coincidence Counting 5.6 Proper Start-up Procedure 6 Alignment 6.1 Violet Beam Path 6.2 Infrared Bea...

This paper describes the construction of a laser transceiver designed specifically for the ARRL 10-GHz-and-Up contest. Earlier versions of this transceiver were presented in Microwave Update Proceedings for 1998 through 2000. This version has been greatly simplified. The previous amplifier incorporated automatic gain control to prevent saturation, which was found unnecessary. Also, the detector...

Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coup...

Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime. Here, a 32 × 1 single-photon avalanche diode (SPAD) array was explored as a detector for fluorescence lifetime imaging (FLIM) in HCS. Device parameters and characterization results were interpreted in th...

Fenarimol (Rubigan) is a pyrimidine ergosterol biosynthesis inhibitor used as a systemic fungicide. The authors present a fatal fenarimol intoxication case analysed in the Forensic Toxicology Service of the National Institute of Legal Medicine. The results were used to compare two different HPLC techniques, regarding selectivity and sensitivity: an HPLC system with a diode array detector (DAD) ...