A protocol has been developed for the direct induction of adventitious shoots from leaf explants of in vitro raised Anthemis xylopoda O.Schwarz, a critically endangered Turkish endemic. The Murashige and Skoog (MS) media supplemented with N–benzyladenine (BA), kinetin (KIN), and thidiazuron (TDZ) were used in regeneration experiments. BA at 0.5 mg L concentration was chosen as the optimal treat...

We have developed a spatially inexplicit model of canopy photosynthesis for balsam fir (Abies balsamea (L.) Mill.) that accounts for key processes of light-shoot interaction including irradiance interception by the shoot, spatial aggregation of shoots into branches and crowns, the differential propagation of diffuse and direct light within the canopy, and an ideal representation of penumbra. Al...

A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% su...

Bacopa monnieri (L.) Wettst. was studied for shoot induction and regeneration on Murashige and Skoog (MS) medium supplemented with different plant growth regulators. Stem explants cultured on medium containing 0.1 mg/l thidiazuron (TDZ) resulted in the highest number of shoots (117 shoots/explant). Regenerated plants from medium with 0.5 mg/l TDZ contained the highest level of pseudojujubogenin...

In vitro flowering and fruiting were induced in plants regenerated from calli of leaf explants of Lycopersicon esculentum var. Pant 11. MS medium supplemented with 2 mg/l BAP displayed the best response followed by MS with 2 mg/l BAP, 1 mg/ l ABA and 0.5 mg/l IAA. Flower buds were developed both directly from callus and regenerated plantlets from leaf explants under 16 h (39.3 μmol m-2s-1) phot...

BACKGROUND Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. The current methods for the production of transgenic grape plants are based on Agrobacterium-mediated transformation followed by regeneration from embryogenic callus. However, grape embryogenic calli are laborious to establi...

An efficient genetically stable regeneration protocol with increased phytochemical production has been established for Dendrobium nobile, a highly prized orchid for its economic and medicinal importance. Protocorm like bodies (PLBs) were induced from the pseudostem segments using thidiazuron (TDZ; 1.5 mg/l), by-passing the conventional auxin-cytokinin complement approach for plant regeneration....

Heavy metal contamination of agricultural land is partly responsible for limiting crop productivity. Cd is known as a non-essentiel HM that can be harmful to plants even at low concentrations. Brassica juncea (L.) is able to accumulate more than 400 μg.g D.W. in the shoot, a physiological trait which may be exploited for the phytoremediation of contaminated soils and waters. In our study, we ha...

Chicory (Cichorium intybus L.) is a low-height perennial or biennial herb from the family of Asteraceae. Investigation different in vitro regeneration strategies Cichorium and increasing number secondary metabolites regenerated plant samples were aims research. Callus regenerations achieved basal growth media supplemented with regulators (PGRs). Whole was carried out by direct organogenesis lea...