kin17 has been described as a protein involved in the processes of DNA replication initiation, DNA recombination, and DNA repair. kin17 has been studied as a potential molecular marker of breast cancer. This work reports the detection and localization of this protein in the murine melanoma cell line B16F10-Nex2 and in two derived subclones with different metastatic potential, B16-8HR and B16-10...

Human T-cell leukemia virus type 1 (HTLV-1) Tax affects cellular genomic stability and senescence. As yet, the mechanism(s) for these events caused by Tax is incompletely understood. Here, we show that Tax expression in primary human cells induces reactive oxygen species (ROS), which elicits DNA damage and the expression of senescence marker. Treatment with a ROS scavenger or knockdown of Tax e...

s/). 8. Bhattramakki, D. et al., in Plant and Animal Genome VIII Conference, 9–12 January 2000, San Diego, USA (http://www.intl-pag.org/pag/8/abstracts/). 9. Mohan, M., Nair, S., Bhagwat, A., Krishna, T. G., Yano, M., Bhatia, C. R. and Sasaki, T., Mol. Breed., 1997, 3, 87–103. 10. Gupta, P. K., Varshney, R. K., Sharma, P. C. and Ramesh, B., Plant Breed., 1999, 118, 369–390. 11. Gupta, P. K. and...

The discovery of fetal DNA in the plasma of pregnant women has opened up new approaches for noninvasive prenatal diagnosis and monitoring. Up to now, the lack of a fetal DNA marker that can be universally detected in maternal plasma has limited the clinical application of this technology. We hypothesized that epigenetic differences between the placenta and maternal blood cells could be used for...

Tomato is an important crop and regarded as an experimental model of the Solanaceae family and of fruiting plants in general. To enhance breeding efficiency and advance the field of genetics, tomato has been subjected to DNA marker studies as one of the earliest targets in plants. The developed DNA markers have been applied to the construction of genetic linkage maps and the resultant maps have...

With over 65 million cats in the United States today (Pet Food Institute, Washington, D.C.), a large percentage of U.S. households have a cat and thus an abundance of cat hair. With the appropriate DNA tools, cat hairs could be used by a forensic laboratory to provide a link between the perpetrator of a crime and a crime scene. An assailant may unknowingly carry clinging cat hairs from a victim...

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Intragenomic variation is the molecular within genome among repetitive DNA. As a multigene family, nuclear ribosomal DNA (rDNA) has been widely used in fungal taxonomy for their ease amplification and suitable variability to attain various levels of taxonomic resolution. At intraspecific level, rDNA believed be under concerted evolution internal transcribed spacers (ITS) region actually accepte...

The objective of this study was to investigate polymorphisms MASP2, TG5 and DQA1 genes using PCR-DNA sequencing in seventy Holstein, Brown Swiss dairy cows (35 each). Under complete aseptic condition, blood samples were collected from each animal into tubes containing disodium EDTA as an anticoagulant for DNA extraction. PCR carried out amplification 305-bp 545-bp TG5, 373-bp genes. assessment ...