Oxidative stress is thought to play a role in the pathogenesis of age-related macular degeneration (AMD). We determined the extent of oxidative DNA damage and the kinetics of its removal as well as the genotypes of the Ser326Cys polymorphism of the hOGG1 gene in lymphocytes of 30 wet AMD patients and 30 controls. Oxidative DNA damage induced by hydrogen peroxide and its repair were evaluated by...

Extraction of high-quality and-quantity DNA is a fundamental requirement for genetic research. It is very important to address the use of DNA extraction methods that are simple and cost-effective in gene polymorphism with large number of samples. This study was designed to investigate the optimal DNA extraction from lymphocytic cells by salt-out method. In this study, 200 blood samples of the t...

Introduction: There are debates about the role of X-ray repair cross-complementation group 1 (XRCC1) Arg399Gln gene in pathogenesis Systemic Lupus Erythematosus (SLE). Methods: The study was a case-control carried out on 100 recently diagnosed SLE patients compared to control subjects. XRCC1 polymorphism performed by polymerase chain reaction and restriction fragment length polymorphism. Result...

Lancet liver flukes of the genus Dicrocoelium (Trematoda: Digenea) are recognised parasites domestic and wild herbivores. The aim present study was to confirm species identity Dicrocoeliid collected from Chitral valley in Himalayan ranges Pakistan. morphology 48 belonging eight host populations examined; but overlapping traits prevented accurate designation. Phylogenetic comparison published D....

Introduction The human RAD52 protein plays an important role in DNA homologous recombination and DNA double-strand break repair. BRCA1 and BRCA2 are involved in the DNA damage response pathway shared with RAD51 and RAD52 (1). Bell et al. (2) observed a germ-line Ser346ter truncation polymorphism (TCG to TAG), resulting in removal of 72 COOHterminal residues, in 3 of 99 cases ( 40 years old) wit...

AIM The paper focuses on candidate gene polymorphism and on the role of dopamine transporter gene polymorphism DAT 1 in the pathogenesis of alcoholism. We investigated this polymorphism in the association study in a whole group of alcoholics (n = 150), fathers (n = 84) and mothers (n = 101) and patients with alcohol dependence (n = 103). The control group consisted of healthy volunteers with ex...

We have developed DNA-based genetic markers for rapid cycling Brassica rapa (RCBr), also known as Fast Plants. Although markers for B. rapa already exist, ours were intentionally designed for use in a teaching laboratory environment. The qualities we selected for were robust amplification in PCR, polymorphism in RCBr strains, and alleles that can be easily resolved in simple agarose slab gels. ...

The population structure of Phytophthora capsici among asexual and sexual progenies was analyzed using ISSR. Thirty one parent three populations were assayed for genetic diversity 5 ISSR primers DNA from 120 offspring P. amplified. In total, 71 reproducible fragments obtained, which 100% polymorphic, revealing high polymorphism the isolates. Among them, percentages progeny isolates 100.00 77.46...

In the human genome, short tandem repetitive (STR) DNA sequences often show restriction fragment length polymorphisms (RFLPs) due to variation in the number of copies of the repeat unit. For a subset of these sequences known as minisatellites or variable number tandem repeat loci (VNTR), it has been proposed that a homologous "core" sequence of 10-12 nucleotides is involved in the mechanism(s) ...

DNA repair capacity (DRC) is correlated with sensitivity of cancer cells toward platinum-based chemotherapy. We hypothesize that genetic polymorphisms in DNA repair gene XPA (xeroderma pigmentosum group A) and XPG (xeroderma pigmentosum group G) (ERCC5, excision repair cross-complementation group 5), which result in inter-individual differences in DNA repair efficiency, may predict clinical res...