A Dynamic Nuclear Polarisation (DNP) enhanced solid-state Magic Angle Spinning (MAS) NMR spectrometer which uses a 187 GHz (corresponding to (1)H NMR frequency of 284 MHz) Extended Interaction Klystron (EIK) amplifier as the microwave source is briefly described. Its performance is demonstrated for a biomolecule (bacteriorhodopsin), a pharmaceutical, and surface functionalised silica. The EIK i...

T statistics are staggering and cited often: Between 2000 and 2030, the U.S. population of older adults is projected to double as the Baby Boomer generation turns 65 (He, Sengupta, Velkoff, & DeBarros, 2005). These increased numbers of older adults bring more complex health care needs, including startling epidemics of obesity and diabetes, and higher acuity during a nursing shortage that is onl...

In aerobic conditions, the heart preferentially oxidizes fatty acids. However, during metabolic stress, glucose becomes the major energy source, and enhanced glucose uptake has a protective effect on heart function and cardiomyocyte survival. Thus abnormal regulation of glucose uptake may contribute to the development of cardiac disease in diabetics. Ketone bodies are often elevated in poorly c...

Erik Martin’s passion for nursing ignited after his first daughter was born 11 weeks prematurely, and he inspired to pursue a career in seeing the incredible difference nurses caring made. In 2000, graduated with diploma from The Good Samaritan School of Nursing Cincinnati, Ohio. began as pediatric float pool nurse then obtained bachelors science degree Mount St. Joseph University 2005, masters...

Herein, we investigate a novel set of polarizing agents—mixed-valence compounds—by theoretical and experimental methods demonstrate their performance in high-field dynamic nuclear polarization (DNP) NMR experiments the solid state. Mixed-valence compounds constitute group molecules which molecular mobility persists even solids. Consequently, such agents can be used to perform Overhauser-DNP sta...

Although dynamic nuclear polarization (DNP) has dramatically enhanced solid-state NMR spectral sensitivities of many synthetic materials and some biological macromolecules, recent studies of membrane-protein DNP using exogenously doped paramagnetic radicals as polarizing agents have reported varied and sometimes surprisingly limited enhancement factors. This motivated us to carry out a systemat...

The ease of tolerance induction in B lymphocytes from fetal, neonatal, and adult mice was studied in vivo, in a cell transfer system, and in vitro. Three different tolerogens were used: ultracentrifuged BGG, DNP(6)-D-GL, and ultracentrifuged DNP(22)-BGG. Irradiated thymectomized mice were reconstituted with B cells from fetal or neonatal liver or adult spleen or bone marrow. The mice were injec...

At the Annual Conference of the National Association of Pediatric Nurse Practitioners held March 1922, 2009, in San Diego, California, Drs. Arlene Sperhac and Patricia Clinton presented a session, ‘‘The DNP and Unintended Consequences: An Opportunity for Dialogue,’’ to address issues that have emerged as a result of the shift in advanced practice education and the opening of Doctor of Nursing P...

The ability of the liver and small intestine for secretion of dinitrophenyl-S-glutathione (DNP-SG), a substrate for multidrug resistance-associated protein 2 (Mrp2), into bile and lumen, respectively, as well as expression of Mrp2 in both tissues, were assessed in 70-75% hepatectomized rats. An in vivo perfused intestinal model was used. A single i.v. dose of 30 micromol/kg b.w. of 1-chloro-2,4...

Rabbit anti-2,4-dintrophenyl (DNP) antibodies or their F(ab')2 fragments were chemically cross-linked to the anti-mouse Fc gamma R monoclonal antibody 2.4G2 or to its Fab fragment. P388D1 cells were incubated with heteroaggregates between 2.4G2 and anti-DNP (anti-Fc gamma R X anti-DNP) and washed. The resulting cells lysed 2,4,6-trinitrophenyl chicken erythrocytes (TNP CRBC) in a hapten-specifi...