Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is a powerful technique used for separation, detection and quantification of complex protein mixtures expressed within cellular systems. Whereas a single-dimension electrophoretic separation allows to detect individual proteins in cell extracts mainly based on a single physicochemical parameter only and may be therefore inadequate for...

Double-exchange model in infinite dimension is studied as the strong Hund’s coupling limit J → ∞ of the Kondo lattice model. Several quantities such as Green’s function and the d.c. conductivity are calculated in analytical forms. Magnetoresistance in lightly doped La1−xSrxMnO3 is reproduced very well.

The synthesized cobalt(II) complex, CoPNBDENA and the binding of this complex with sheep genomic DNA were investigated by UV–Visible absorption and viscosity techniques. Also the interaction of sheep genomic DNA with the complex was studied using the agarose gel electrophoresis method. The results indicated that the complex interacted with DNA. The nature of the binding seemed to be mainly an e...

Two human associative-learning experiments investigated the relationship between the negative dimension of schizotypy and selective and nonselective prediction-error learning. Experiment 1 demonstrates that individuals low, but not high, on the introvertive anhedonia dimension of schizotypy demonstrate Kamin blocking, which has been taken to reflect the operation of selective learning (Rescorla...

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has ...

New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electrophoresis. These tiles' purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays vis...

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (10...

The relative lability of the interchain disulphide bonds of mouse G(2a)-myeloma protein 5563 was studied as a function of 2-mercaptoethanol concentration. Analysis of partial-reduction mixtures by polyacrylamide-gel electrophoresis and microdensitometry showed that the disulphide bonds between light and heavy chains are much more susceptible to reduction than the bonds between heavy chains. At ...