(1) Background: Mixotrophic growth is commonly associated with higher biomass productivity and lower energy consumption. This paper evaluates the impact of using different carbon sources on growth, protein profile, nutrient uptake for Dunaliella tertiolecta CCAP 19/30 to assess potential mixotrophic growth. (2) Methods: Two experimental sets were conducted. The first assessed contribution atmos...

Dunaliella tertiolecta required carbon dioxide in substrate concentrations (1.75 %, v/v) to assimilate either nitrate or nitrite at maximum rates in light. The addition of glucose, glycerol, acetate, pyruvate or a-ketoglutarate did not remove the requirement for carbon dioxide. The rates of nitrate and nitrite assimilation in light depended upon the buffer system used. The lowest rates of nitra...