نتایج جستجو برای: duplex rt pcr
تعداد نتایج: 214870 فیلتر نتایج به سال:
widespread occurrence of h9n2 low pathogenic avian influenza (lpai) viruses in many asian countriesduring the past decade has resulted in the need for evaluation of the pathogenesis of h9n2 virus infection. in this study, tissue tropism and dissemination of a/chicken/iran/772/1998(h9n2) virus throughout the body of broiler chickens were investigated. the clinical signs, gross lesions and antibo...
گیاه ocimum basilicum l. از خانواده نعناعیان است که دارای ترکیبات حلقوی و اسانس بوده و خواص ضد باکتریایی و آنتی اکسیدانی دارد، که سرشار از ترکیبات فنیل پروپانوئیدی است. آنزیم فنیل آلانین آمونیالیاز یکی از آنزیمهای کلیدی در مسیر تولید ترکیبات فنیل پروپانوئیدی میباشد. شواهد نشان میدهد که هورمونهای گیاهی بر بیان ژن در گیاهان مؤثر بوده و موجب افزایش تولید متابولیتهای ثانویه میگردند. علاوه بر...
One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...
The objective of this study is to compare and assess the effectiveness real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), selective agar plate method for detection Salmonella spp. Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, performance three methods (RT-PCR [SureTectTM kit PowerChekTM kit], LAMP [3M MDS], agar) were similar at 0-10...
Melissococcus plutonius is the causative agent of an important honeybee disease, European foulbrood (EFB). In addition to M. plutonius strains with typical characteristics (typical M. plutonius), we recently reported the presence of atypical M. plutonius, which are phenotypically and genetically distinguished from typical M. plutonius. Because typical and atypical M. plutonius may have differen...
A duplex real-time PCR assay was designed for simultaneous detection and genotyping of Mycoplasma pneumoniae (M. pneumoniae). The detection/typing performance of this duplex PCR method, targeting specific genes for M. pneumoniae type 1 (mpn 459) and type 2 (mpna 5864), was compared to that of the previously published MpP1 real-time PCR assay and the genotyping method for the adhesin P1 gene (mp...
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