INTRODUCTION Today, with the tremendous potential of genomics and other recent advances in science, the role of science to improve reliable DNA extraction methods is more relevant than ever before. The ideal process for genomic DNA extraction demands high quantities of pure, integral and intact genomic DNA (gDNA) from the sample with minimal co-extraction of inhibitors of downstream processes. ...

Pseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for beta-1,3 glucanase production. From the culture filtrate, beta-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its beta-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for beta-1,3 glucanase activity on laminarin was found to be 7 and 50 deg...

This study assesses the effect of the superoxide anion scavenger superoxide dismutase on myocardial capillary permeability-surface area (PS) products for small hydrophilic molecules after ischemia and reperfusion. Open-chest dogs underwent a 20-minute occlusion of the left anterior descending coronary artery followed by 1 hour of reperfusion. Myocardial plasma flow rate and capillary extraction...

This study assesses the effect of the superoxide anion scavenger superoxide dismutase on myocardial capillary permeability-surface area (PS) products for small hydrophilic molecules after ischemia and reperfusion. Open-chest dogs underwent a 20-minute occlusion of the left anterior descending coronary artery followed by 1 hour of reperfusion. Myocardial plasma flow rate and capillary extraction...

The myocardial transmicrovascular transport of thallium-201 (201Tl) and technetium-99m hexakis(2-methoxyisobutylisonitrile) (MIBI) were compared during variable blood flow levels in nine blood-perfused, isolated rabbit hearts. Seventeen injections of radiolabeled albumin and EDTA as well as 201Tl and MIBI were performed by indicator-dilution techniques. When coronary flow was varied from 0.52 t...

Ancient DNA analyses rely on the extraction of the tiny amounts of DNA remaining in samples that are hundreds to tens of thousands of years old. Despite the critical role extraction efficiency plays in this field of research, no study has comprehensively compared ancient DNA extraction techniques to date. There are a wide range of methods currently in use, which rely on such disparate principle...

OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...

Ethylenediaminetetraacetic acid (EDTA) was developed by Franz Munz inGermanyduring the 1930s as an alternative to citric acid. About the same time, Frederick Bersworth synthesized EDTA in the United States using a different technique. He obtained aUSpatent for this technique in1945. EDTAwas approvedby the US Food and Drug Administration (FDA) as a food additive in 1947. Since the early 1950s, E...

Brucellosis is a zoonotic bacterial disease with worldwide distribution. A cross-sectional study was conducted to determine the serological prevalence of brucellosis in horses within Kano Metropolis. total 328 serum samples were collected for study. Modified Rose Bengal Plate Test (mRBPT) on all while Serum Agglutination EDTA (SAT-EDTA) mRBPT-positive samples. The metropolis found be 24.09 % (7...