OBJECTIVE Uptake of modified low-density lipoprotein (LDL) by macrophages through scavenger receptors results in lipid droplets accumulation and foam cell formation. Excess lipid deposition in macrophages has been reported to modulate expression of several genes including adipophilin. In this study, we investigated the function of adipophilin in lipid accumulation and cholesterol efflux in THP-...

Enterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. Management of these infections is often difficult, owing to the high frequency of strains that are resistant to disinfectants and antimicrobial agents in the clinical setting. Multidrug efflux pumps, especially those belonging to the resistance-nodulation-division family, play a major role a...

The genus Burkholderia comprises rod-shaped, non-spore-forming, obligately aerobic Gram-negative bacteria that is found across diverse ecological niches. contaminans, an emerging pathogen associated with cystic fibrosis, frequently isolated from contaminated medical devices in hospital settings. aim of this study was to understand the genomic characteristics, antimicrobial resistance profile an...

The existence of antibiotic-resistant bacteria in food products, particularly those carrying acquired resistance genes, has increased concerns about the transmission these genes from beneficial microbes to human pathogens. In this study, we evaluated antibiotic resistance-susceptibility patterns 16 antibiotics eight S. thermophilus strains, whose genome sequence is available, using phenotypic a...

The potential use of microorganisms in the bioremediation U pollution has been extensively described. However, a lack knowledge on molecular resistance mechanisms become challenge for these technologies. We reported transcriptomic and microscopic response Stenotrophomonas bentonitica BII-R7 exposed to 100 250 ?M U. Results showed that exposure displayed up-regulation 185 148 genes during lag ex...

Active efflux from procaryotic as well as eucaryotic cells strongly modulates the activity of a large number of antibiotics. Effective antibiotic transport has now been observed for many classes of drug efflux pumps. Thus, within the group of primary active transporters, predominant in eucaryotes, six families belonging to the ATP-binding cassette superfamily, and including the P-glycoprotein i...

A critical event in the development of atherosclerosis is the recruitment of macrophages to the underlying epithelial layer of blood vessel walls and the uncontrolled uptake of oxi-dized/modified cholesterol. Continued accumulation of oxi-dized/modified cholesterol by macrophages and an associated inflammatory response leads to foam cell formation and the initiation of atherosclerosis. 1 Revers...

The drug resistance of Vibrio harveyi in aquaculture became more severe because the control Vibriosis depends majorly on current antibiotics. Transcriptomes a wild-type strain (VS) and its quinolone-resistant mutants (VR) V. were respectively sequenced by RNA-seq technology. A total 2,082 unigenes obtained after de novo splicing assembly. 129 genes identified with significant differential expre...

Malate and citrate efflux from root apices is a mechanism of Al(3+) tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al(3+)-sensitive cereal species but the small genotypic variation in tolerance that is present is correl...

Inactivation of the Escherichia coli gene ydeA, which encodes a member of the major facilitator superfamily, decreased the efflux of L-arabinose, thereby affecting the expression of AraC-regulated genes. In addition, overexpression of ydeA decreased the expression of genes regulated by isopropyl-beta-D-thiogalactopyranoside.