RITCHIE, A. E. (National Animal Disease Laboratory, Ames, Iowa), AND HERMAN C. ELLINGHAUSEN. Electron microscopy of leptospires. I. Anatomical features of Leptospira pomona. J. Bacteriol. 89:223-233. 1965.-Anatomical features of Leptospira pomona are described. Most cells in the logarithmic phase of growth, when examined in whole cell "negative-stained" preparations, display a homogeneous finel...

Some uses of the computer and computation in high-resolution transmission electron microscopy are reviewed. The theory of image calculation using Bloch wave and multislice methods with and without aberration correction is reviewed and some applications are discussed. The inverse problem of reconstructing the specimen structure from an experimentally measured electron microscope image is discuss...

In a previous communication (1), one of us described the propensity for emperipolesis exhibited by the cells of the mouse ascites lymphocytic tumor, Lymphoma No. 1 (L, 1). Emperipolesis, a term coined by Humble, Jayne, and Pulvertaft (2) to describe the "inside round about wandering" of lymphocytes within cells, has been observed by several investigators to occur under a variety of conditions (...

It is widely acknowledged that EM of macromolecules is entering a new phase of productivity (for review see Nogales and Grigorieff, 2001). It is less well known that comparable innovations are afoot in cellular EM. Studies of biological “ultra-structure” were of key importance in the early years of cell biology, but more recently their importance has waned significantly. This change has been la...

THE ALDEHYDES INTRODUCED IN THIS PAPER AND THE MORE APPROPRIATE CONCENTRATIONS FOR THEIR GENERAL USE AS FIXATIVES ARE: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylate- or phosphate-buffered solutions (0.1 to 0....

Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the strong atomic scattering, and the ability to examine tiny volumes of matter (≈1...

introduction: presence of surface defects in endodontic instruments can lead to unwanted complications such as instrument fracture and incomplete preparation of the canal. the current study was conducted to evaluate the effect of autoclave cycles on surface characteristics of s-file by scanning electron microscopy (sem). methods and materials: in this experimental study, 17 brand new s-files (#...

in this work, the effects of acetic and lactic acids addition to rice during cooking, on the texture and morphological properties, and too, the gelatinization temperature (gt) of the cooked domsiyah rice was investigated. all experiments were carried out in triplicate and data analyzed in randomized completely by spss15 software. the results showed that acid addition to rice during cooking caus...

sol–gel auto-combustion is a unique combination of the combustion and the chemical gelation processes. in this work, nanosize (d) powders with compositions of znfe2o4 were synthesized by sol–gel combustion method. the puffy, porous brown powders as-combusted was calcined at the temperature of 750–1000◦c for 4 h. these powders are characterized by x-ray diffraction and scanning electron microsco...