In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures...

We modified a time-lapse recording to make a ‘flipbook’ of zebrafish embryogenesis. By flipping through the pages of this issue and watching the pictures in the upper corner of each even-numbered page, the dynamic cellular movements that form the zebrafish embryo can be visualized. The time-lapse record starts at the 2-cell stage (page 2 and Fig. 1B) and ends at the 16-somite stage (Fig. 1I), a...

background: high dietary fat consumption may alter oocyte development and embryonic development. this prospective study was conducted to determine the relation between dietary fat consumption level, its food sources and the assisted reproduction parameters. methods: a prospective study was conducted on 240 infertile women. in assisted reproduction treatment cycle, fat consumption and major food...

The formation of dorsal-ventral polarity in Drosophila requires the asymmetric nuclear localization of the dorsal protein along the D/V axis. This process is regulated by the action of the dorsal group genes and cactus. We show that dorsal and cactus are both phosphoproteins that form a stable cytoplasmic complex, and that the cactus protein is stabilized by its interaction with dorsal. The dor...