Hydrolysis of Thiamine Pyrophosphate (TPP) is too sluggish even at elevated temperatures in aqueous buffer media. However, the hydrolysis reactions of thiamine pyrophosphate (TPP) are dramatically accelerated under micellar conditions even at room temperature by the addition of anionic (SDS) and nonionic (Tx) micelles. Menger – Portnoy’s enzymatic model and Piszkiewicz co-operativity model were...

Up to now, experimental limitations have prevented researchers from achieving the molecular-level understanding for the initial steps of the enzymatic hydrolysis of cellulose, where cellulase breaks down the crystal structure on the surface region of cellulose and exposes cellulose chains for the subsequent hydrolysis by cellulase. Because one of these non-hydrolytic enzymatic steps could be th...

To change the recalcitrant nature of the lignocellulosic material for maximum hydrolysis yield, a comprehensive study was done by using sulphuric acid as an exclusive catalyst for the pretreatment process. The enzymatic digestibility of the biomass [Water Hyacinth: Eichhornia crassipes] after pretreatment was determined by measuring the hydrolysis yield of the pretreated material obtained from ...