The biological activity of the immobilized enzyme is crucial for the performance of different nanoparticle mediated enzymatic assays, where enzymatic conversion can be used for label-free analyte detection. In this article we have addressed two significant aspects of enzyme-nanoparticle interactions. First, we have developed copper sulfide (CuS) nanoparticles with an average diameter of 25 nm a...

Cascade reactions catalyzed by immobilized multi enzymatic systems are emerging as a tool to address the increasing need for green and sustainable chemistry. In this regard, carrier-free immobilization strategy of combined cross-linked enzymes aggregates (combi- CLEAs) is great interest. combi-CLEAs two or more co-immobilized into single unit where they accommodated in close proximity order min...

Enzymatic detection of organophosphate (OP) compounds can be tailored using highly sensitive and selective enzymes in the development biosensors. Previously, mutant (YT) phosphotriesterase (PTE) was reported to efficiently hydrolyze Sp Rp enantiomers phosphotriester. This study reports use YT (YT-PTE) immobilized onto reduced graphene oxide (rGO) fabricated a screen-printed carbon electrode (SP...

The industrial and environmental applications of laccase, especially in wastewater treatment, have gained focus recent years. Therefore, developing the proper laccase immobilization techniques, which could improve stability enzymes simplify required downstream processes, is needed. A novel two-step process was developed, resulting cross-linked enzyme aggregates (CLEA) pores membrane. Laccase ad...

Penicillinase-modified AlGaN/GaN field-effect transistors (PenFETs) are utilized to systematically investigate the covalently immobilized enzyme penicillinase under different experimental conditions. We demonstrate quantitative evaluation of covalently immobilized penicillinase layers on pH-sensitive field-effect transistors (FETs) using an analytical kinetic PenFET model. This kinetic model is...

Enzyme immobilization provides substantial advantages in terms of improving the efficiency enzymatic process as well enhancing reusability enzymes. Phasins (PhaPs) are naturally occurring polyhydroxyalkanoate (PHA)-binding proteins, and thus can potentially be used a fusion partner for oriented enzymes onto PHA supports. However, presently available granular supports have low surface-area-to-vo...

A leading biotechnological advancement in the field of biocatalysis is immobilization enzymes on solid supports to create more stable and recyclable systems. Metal-organic frameworks (MOFs) are porous materials that have been explored as for enzyme immobilization. Composed organic linkers inorganic nodes, MOFs feature empty void space with large surface areas ability be modified post-synthesis....

The overall objective of this research is to enhance the bioactivity of cellulase enzymes as well as the stability of the enzymes to increase it reusability through immobilization of the enzymes in the microenvironment of porous inorganic-organic hybrid sol-gel polymers. Recently, we have discovered that the microenvironment of these sol-gel polymers markedly increases the bioactivity of immobi...