Though lacking a well-defined three-dimensional structure, intrinsically unstructured proteins are ubiquitous in nature. These molecules play crucial roles in many cellular processes, especially signaling and regulation. Surprisingly, even enzyme catalysis can tolerate substantial disorder. This observation contravenes conventional wisdom but is relevant to an understanding of how protein dynam...

The multistep differential transform method is first employed to solve a time-fractional enzyme kinetics. This enzyme-substrate reaction is formed by a system of nonlinear ordinary differential equations of fractional order. The fractional derivatives are described in the Caputo sense. A comparative study between the new algorithm and the classical Runge-Kutta method is presented in the case of...

objective(s): streptococcus pyogenes produces extracellular hyaluronidase enzyme. this enzyme is directly associated with the spread of the organism during infection. the objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. materials and methods: the enzymatic region of hyaluronidase gene was detecte...

Optimization of Enzymatic saccharification conditions for acid treated bmr sorghum biomass was studied with the inhouse enzyme produced from Aspergillus sp.. Optimization of enzyme loading, substrate concentration, temperature and time yielded a maximum of 416.00 ± 0.55 mg/g sugars with an improved hydrolysis efficiency of 55.97 ± 0.45%. Protein adsorption onto lignocellulosic substance was inv...

The role of protein dynamics in the reaction catalyzed by dihydrofolate reductase from the hyperthermophile Thermotoga maritima (TmDHFR) has been examined by enzyme isotope substitution ((15)N, (13)C, (2)H). In contrast to all other enzyme reactions investigated previously, including DHFR from Escherichia coli (EcDHFR), for which isotopic substitution led to decreased reactivity, the rate const...

This study focused on defining the differences in L. plantarum gene expression levels in different media and in different growth phases using an easy and cost-efficient monitoring of gene expression. A macroarray based on a group of selected L. plantarum genes, 178 genes belonging to 18 main groups, printed onto a nitrocellulose filter was designed in this work. Using the macrofilters designed,...

Enzymes within biochemical pathways are often colocalized, yet the consequences of specific spatial enzyme arrangements remain poorly understood. We study the impact of enzyme arrangement on reaction efficiency within a reaction-diffusion model. The optimal arrangement transitions from a cluster to a distributed profile as a single parameter, which controls the probability of reaction versus di...

We report the synthesis and characterization of a water-stable zirconium metal-organic framework (MOF), NU-1003, featuring the largest mesoporous aperture known for a zirconium MOF. This material has been used to immobilize the nerve agent hydrolyzing enzyme, organophosphorus acid anhydrolase (OPAA). The catalytic efficiency of immobilized OPAA in nanosized NU-1003 is significantly increased co...

Thermostabilizing an enzyme while maintaining its activity for industrial or biomedical applications can be difficult with traditional selection methods. We describe a rapid computational approach that identified three mutations within a model enzyme that produced a 10 degrees C increase in apparent melting temperature T(m) and a 30-fold increase in half-life at 50 degrees C, with no reduction ...

Gram-positive bacteria are some of the earliest known life forms, diverging from gram-negative 2 billion years ago. These organisms utilize sortase enzymes to attach proteins their peptidoglycan cell wall, a structural feature that distinguishes two types bacteria. The transpeptidase activity sortases make them an important tool in protein engineering applications, e.g., sortase-mediated ligati...