1. An enzyme that catalyzes the formation of fOrmyl-Dkynurenine from D-tryptophan was purified about loo-fold from a homogenate of rabbit small intestine. Formyl-~kynurenine thus produced was converted to D-kynurenine by formamidase present in rabbit intestine. 2. Methylene blue and ascorbic acid were required as cofactors. Xanthine oxidase with hypoxanthine could replace ascorbic acid, but hyd...

In the framework of artificial or synthetic cell development, giant liposomes are common basic structures. Their enclosed membrane allows encapsulating proteins, DNA, reactants, etc., while its phospholipid nature some exchanges with surrounding medium. Biochemical reactions induced inside vesicles often monitored imaged by fluorescence microscopy techniques. Here, we show that electrochemistry...

Trends in biosensor research and development are described. Micromachining techniques have been applied to construct biosensor systems. Several detection units for flow injection analysis have been fabricated. An electrochemical flow cell was fabricated, and both an enzyme immobilized column and an electrochemical detector were integrated. A chemiluminescence detector was also fabricated. Biose...

the surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. in this study, conformational changes of glucose oxidase (gox) in the mercaptopurine: gnps and 11-mercaptoundecanoic acid: gnps as a hydrophobic and a hydrophilic gnps surface was investigated by various spectroscopic techniques, including: uv-vis absorption, f...

Three types of molecular systems for insulin release in response to glucose were designed and synthesized. i) Chemical valve was synthesized by immobilization of glucose oxidase onto poly(acry1ic acid)-grafted porous membrane. ii) Membrane device was constructed by immobilization of insulin through disulfide linkage with combination of glucose dehydrogenase and electron mediators. iii) Enzyme d...

in this paper, multi-wall carbon nanotubes (mwcnts), gold nanoparticles (gnp) and glucose oxidase (god) was developed for the specific detection of glucose. mwcnts were chemically modified with the h2so4–hno3 pretreatment to introduce carboxyl groups which were used to interact with the amino groups of poly(allylamine) (paa) and cysteamine via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/n-hy...

The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp. in a 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. The effect of submerged ...

Pyranose 2-oxidase (P2O) from Trametes multicolor is a flavoprotein oxidase that catalyzes the oxidation of aldopyranoses by molecular oxygen to yield the corresponding 2-keto-aldoses and hydrogen peroxide. P2O is the first enzyme in the class of flavoprotein oxidases, for which a C4a-hydroperoxy-flavin adenine dinucleotide (FAD) intermediate has been detected during the oxidative half-reaction...

Introduction Sugar oxidases and dehydrogenases that catalyze carbohydrate oxidation into the corresponding lactones are of considerable commercial importance. Glucooligosaccharide oxidase (GOOX) from Acremonium strictum was screened with the aim of identifying enzymes with potential applications such as an oligosaccharide acid production and various alternative carbohydrate assays. Screening of...

A new flavooxidase is described from a Bjerkandera arthroconidial anamorph. Its physicochemical characteristics, a monomeric enzyme containing non-covalently bound flavin adenine dinucleotide (FAD), and several catalytic properties, such as oxidation of aromatic and polyunsaturated aliphatic primary alcohols, are similar to those of Pleurotus eryngii aryl-alcohol oxidase (AAO). However, it also...