in this study, the effects of dietary egg lecithin on digestive enzymes and body biochemical composition of juveniles mesopotamichthys sharpeyi was evaluated. four experimental diets including control diet (with 0% egg lecithin) and three diets containing 2%, 4% and 6% egg lecithin were used. at the end of the experiment, digestive enzymes activity (lipase, amylase and alkaline phosphatase) and...

the main purpose of this project is investigation of the organophosphorus hydrolase (oph) enzyme activity in water in oil (w/o) and oil in water (o/w) creams and investigation of the oph enzyme stability in formulated creams. oph enzyme was extracted and purified from strain flavobacterium. the w/o and o/w creams were prepared using different formulations. in order to achieve an emulsion with m...

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

Amylase is an important and indispensable enzyme that plays a pivotal role in the field of biotechnology. It is produced mainly from microbial sources and is used in many industries. Industrial sectors with top-down and bottom-up approaches are currently focusing on improving microbial amylase production levels by implementing bioengineering technologies. The further support of energy consumpti...

Proteases are catabolic enzymes that catalyze the complete hydrolysis of protein. They constitute one of the most important groups of industrial enzymes, accounting for nearly 60% of the total worldwide enzyme sale1. Keratinolytic protease is a specific protease that has immense commercial importance. It acts on keratin of the hides thus can be used in dehairing2. This enzyme along with proteas...

Manuscript describes producing keratin hydrolysate from sheep wool through two-stage technology whose principle consists in first having wool processed in an alkaline environment during the first stage, and then effecting hydrolysis in the second stage through the action of proteolytic enzyme. Experiments were planned in accordance with factor schemes of 23 types in which factors under study we...

We report a novel platform technology of enzyme assay using microfluidic channel. Employing the patterns of microfluidic channel on a plastic chip, the dilution of substrate solution and enzyme reactions are sequentially carried out in microfluidic channels. The microfluidic device is fabricated by micromolding processes with polydimethylsiloxane (PDMS) polymer. The device consists of microflui...

The biosensor field has grown enormously since the first demonstration of the biosensor concept by Leland C. Clark Jr. in 1962. Today’s biosensor market is dominated by glucose biosensors, mass-produced enzyme-electrodes for the rapid self-diagnosis of blood glucose levels by diabetes sufferers. Here we take a historical look at the inception, growth, and development of the enzyme biosensor fie...

Objective: Acute chest pain is an important and frequently occurring symptom in patients. Chest pain is often a sign of ischemic heart disease. Chest pain due to suspected Acute Coronary Syndrome (ACS) is responsible for a large and ijncreasing number of hospital attendances and admissions. Current practice for suspected ACS involves troponin testing 10–12 hours after symptom o...

Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme.  For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present.  Based...