OBJECTIVE To develop a quantitative PCR method for detecting hookworm infection and quantification. METHODS A real-time PCR method was designed based on the intergenic region II of ribosomal DNA of the hookworm Necator americanus. The detection limit of this method was compared with the microscopy-based Kato-Katz method. The real-time PCR method was used to conduct an epidemiological survey o...

The membrane-bound [NiFe]-hydrogenase of Cupriavidus necator is a rare example truly O 2 -tolerant hydrogenase. It catalyzes the oxidation H into e – and + in presence...

Background and Aims: Gold nanoparticles have potential applications in the areas of medicine, target drug delivery, cancer diagnosis and therapy, electronic, etc. Recently, biological system is considered as an environmental friendly method for synthesis of stable nanoparticles. Methods: We demonstrated a biological system for formation of stable gold nanoparticle by using Cupriavidus necator w...

Because of their lower environmental impact, biodegradable polymers such as polyhydroxyalkanoates (PHAs) produced within cultured bacteria represent promising alternatives to petroleum-based plastics. PHA production in flasks yielded optimal results with a carbon-to-nitrogen ratio 22. The 5-L scale experimental revealed that when glucose was used the carbon source, Cupriavidus necator could pro...

We conducted a community cross-sectional survey of soil-transmitted helminthiasis in humans and dogs in four provinces in northern Laos. We collected and tested human and dog fecal samples and analyzed results against sociodemographic data. The prevalence of Ascaris lumbricoides, Trichuris trichiura, hookworm, and Strongyloides stercoralis was 26.1% (95% confidence interval [CI] = 23.7-28.4%), ...

Richards J. C., Rebnke J. M. & Duce I. R. 1995. In uifro studies oo tbe relative sensitivity to ivermectin of Necator americanus and AncyIostoma ceyhnicum. International Journal for Parasitology 25: 1185-1191. Experiments were carried out to compare tbe sensitivity of Ancylostoma ceyhnicum and Necator umericunus to ivermectin (IVM) and pyrantel in vitro. Loss of motility and inhibition of inges...

Empirical studies addressing questions of political information and/or sophistication are common and varied. Some assume the importance of an informed citizenry, and attempt to gauge the level, distribution, and correlates of political knowledge in the U.S. public (Hyman and Sheatsley, 1947; Kriesberg, 1949; Metzner, 1949; Berelson, et al., 1954; Hero, 1959; Withey, 1962; Erskine, 1962; 1963a; ...