In order to gain insight into the gene expression profiles associated with anterior regeneration of the earthworm, Perionyx excavatus, we analyzed 1,159 expressed sequence tags (ESTs) derived from cDNA library early anterior regenerated tissue. Among the 1,159 ESTs analyzed, 622 (53.7%) ESTs showed significant similarity to known genes and represented 338 genes, of which 233 ESTs were singleton...

To obtain a primary overview of gene diversity and expression pattern in Lycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were sequenced and 4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967 contigs and 1,343 singlets were obtained. Blast search showed that 179 contigs and 227 singlets (totally 1,066 ESTs) had homologues ...

The problem of estimating discovery probabilities originated in the context of statistical ecology, and in recent years it has become popular due to its frequent appearance in challenging applications arising in genetics, bioinformatics, linguistics, designs of experiments, machine learning, etc. A full range of statistical approaches, parametric and nonparametric as well as frequentist and Bay...

A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation...

This study presents a survey of general characteristics of empirically supported treatments (ESTs) identified by the American Psychological Association Division 12 Task Force on the Promotion and Dissemination of Psychological Procedures. Results indicate that the ESTs share the following characteristics: they involve skill building, have a specific problem focus, incorporate continuous assessm...

The taxonomic status of Pinus henryi, a rare species endemic to China, is still ambiguous. In this study, the genetic relationships among P. henryi and its congeners (P. tabulaeformis, P. tabulaeformis var. mukdensis, and P. massoniana) were revealed using multiplexed microsatellite markers, including chloroplast microsatellites, nuclear microsatellites, and expressed sequence tag microsatellit...

To investigate Saccharomyces cerevisiae 3'-end-processing signals, a set of 1352 unique pre-mRNA 3'-end-processing sites, corresponding to 861 different genes, was identified by alignment of expressed sequence tag sequences with the complete yeast genome. Nucleotide word frequencies in the vicinity of the cleavage sites were analyzed to reveal the signal element features. In addition to previou...

Objective Learning collaboratives (LCs) have potential to promote implementation of empirically supported treatments (ESTs) in healthcare organizations and systems by building networks among clinicians and with key content experts that facilitate shared expertise. However, the effectiveness of LCs for facilitating clinicians’ access to expertise, and implementation success is untested. This stu...

Although treatment manuals have existed for many years, controversy surrounds their use and dissemination. In a previous article, we addressed assets and liabilities of using empirically supported treatments (ESTs) in psychotherapy, as well as obstacles to disseminating such treatments. In this article, we argue that using EST aids clinical work and is ethically sound. At the same time, we draw...

This report gives a skeleton description of the d2 algorithm used for the clustering of expressed sequence tags (ESTs) in the wcd program. It describes how the algorithm works and why some design decisions were made. No experimental evidence is reported here. This is subject of ongoing research.