The progress in recombinant DNA technology has made possible the introduction of exogenous genetic material into cells. Gene therapy aims to correct a defective gene, introduce a therapeutic exogenous gene or a counteracting gene into somatic cells without modification of the germ-cell line. The most important technical interests in the field of gene therapy research have pertained to the devel...

ConClusions: Ex-vivo normothermic limb perfusion preserves limb physiology and function for at least 12 hours. Thermography and ICG angiography are valuable tools in the assessment of limb perfusion quality with the advantage of providing an immediate evaluation which allows for the visual identification of perfusion gradients and regions of mal-perfusion. Muscle contraction upon nerve stimulat...

ConClusions: Ex-vivo normothermic limb perfusion preserves limb physiology and function for at least 12 hours. Thermography and ICG angiography are valuable tools in the assessment of limb perfusion quality with the advantage of providing an immediate evaluation which allows for the visual identification of perfusion gradients and regions of mal-perfusion. Muscle contraction upon nerve stimulat...

Although normothermic machine perfusion of donor livers may allow assessment of graft viability prior to transplantation, there are currently no data on what would be a good parameter of graft viability. To determine whether bile production is a suitable biomarker that can be used to discriminate viable from non-viable livers we have studied functional performance as well as biochemical and his...

PurposeCurrent commercially available EVLP solutions are designed to mimic physiological conditions support donor lungs outside of the human body. These have been effective at offering for donated after brain death but they limited in their ability cardiac death. To characterize hemodynamics utilizing altered purpose enhancing lung viability, a research device was devised.MethodsThe (fig. 1) is...

Although the number of lung transplants in Spain is increasing annually, more organs are required to ease waiting lists. Controlled non-heart beating donors (NHBD) (Maastricht III) are a reality at international level, and contribute significantly to increasing donor numbers. In this study, we present our NHBD protocol and the initial experience in Spain using lung grafts from this type of dono...