PURPOSE Results from this and other laboratories strongly suggest that differences in the properties of the ocular media that bathe cells in the anterior and posterior regions of the lens contribute to its normal growth patterns and polarity. The aim of this study was to compare the effects of aqueous and vitreous on the morphology of lens epithelial explants, with particular attention to chang...

Saccharomyces cerevisiae SU50B and Hansenula polymorpha 8/2, both carrying a multicopy integrated guar alpha-galactosidase, have been cultivated in continuous cultures, using various mixtures of carbon sources and cultivation conditions. Both S. cerevisiae SU50B and H. polymorpha 8/2 are stable and produce high levels of extracellular alpha-galactosidase in continuous cultures for more than 500...

Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.g. scurvy). The main interest in this study is that vitamin ...

tions in the pattern o f newly synthesized and secreted proteins. There appeared t o be three main modulations under the influence of the fucopolysaccharide. One occurred o n SDS/PAGE in the area o f low molecular mass proteins (below 46 kDa) and two alterations occurred in the area of high molecular mass proteins of 220 kDa and 180 kDa. These two proteins were identified as fibronectin and thr...

..............................................................................................................................................3 Table of

Objective: To synthesize silver nanoparticles extracellularly using Bacillus sp. isolated from soil sample. Methods: Bacteria capable of synthesizing silver nanoparticles extracellularly were screened by treating culture supernatant with silver nitrate solution. The potential isolate was isolated on nutrient agar medium and identified by Gram staining and biochemical tests. Results: One of the ...

Human lymphocytes were shown to release, in vitro and in the absence of any stimulation, a complex containing DNA. It has also been reported that the release process is unrelated to cell death and is regulated by a homeostatic mechanism. Some properties of the extracellular DNA were investigated. When a phosphorylated precursor was added to the cell-free supernatant, the DNA recovered from the ...

Extracellular matrix (ECM) within the vascular network provides both a structural and regulatory role. The ECM is a dynamic composite of multiple proteins that form structures connecting cells within the network. Blood vessels are distended by blood pressure and, therefore, require ECM components with elasticity yet with enough tensile strength to resist rupture. The ECM is involved in conducti...

Fibrosis, the hallmark of human injuries and diseases such as serious burns, is characterized by excessive collagen synthesis and myofibroblast accumulation. Transforming growth factor-β (TGF-β), a potent inducer of collagen synthesis, has been implicated in fibrosis in animals. In addition to TGF-β, fibroblast growth factor-inducible molecule 14 (Fn14) has been reported to play an important ro...

PURPOSE Articular cartilage homeostasis involves modulation of chondrocyte matrix synthesis in response to mechanical stress (MS). We studied extracellular and intracellular mechanotransduction pathways mediating this response. METHODS We first confirmed rapid up-regulation of the putative chondro-protective cytokine, interleukin (IL)-4, as an immediate response to MS. We then studied the rol...