Previously, TX5179, a disruption mutant of the enterococcal polysaccharide antigen (epa) gene cluster of Enterococcus faecalis strain OG1RF was shown to be attenuated in translocation, biofilm, mouse peritonitis and was more susceptible to polymorphonuclear leukocyte phagocytic killing. Here, wild-type E. faecalis OG1RF and TX5179 strains were tested in a mixed-infection (inoculum, approximatel...

The methyl moiety of ribothymidine in the tRNA of Streptococcus faecalis, Bacillus subtilis, and probably Bacillus cereus is dervied from a 1-carbon folate derivative and not S-adenosylmethionine. Micrococcus luteus (M. lysodeikticus) tRNA appears to be almost devoid of ribothymidine. S. faecalis tRNA lacking ribothymidine can be methylated in vitro with formation of ribothymidine. 5,10-Methyle...

introduction: root canal treatment is based on elimination of intraradicular microorganisms. calcium hydroxide ca(oh) 2 is the most widely used material for canal dressing, however, enterococcus faecalis has been reported to be resistant bacteria in persistent apical periodontitis . the purpose of this study was to evaluate the efficacy of ca(oh) 2 on the elimination of intraluminal and intratu...

introduction: enterococcus faecalis ( e. faecalis ) has the ability to invade the dentinal tubules and resist high ph levels. as a result, calcium hydroxide (ch) is not much effective on this bacterium. in theory, nanoparticle calcium hydroxide (nch) has smaller size and high surface area that enables it to penetrate into the deeper layers of dentin and be more effective on e. faecalis. this in...

Background: Complete microbial eradication from the root canal and 3-dimensional obturation of space are necessary for an efficient procedure. Aim: The current research was conducted to assess antimicrobial effectiveness herbal irrigants Chlorhexidine against Enterococcus faecalis. Materials methods: brain heart infusion (BHI) broth used grow E. faecalis (ATCC) bacterial culture overnight befor...

We examined 215 Enterococcus faecalis isolates and found that neither the two-component regulatory locus fsr (E. faecalis regulator) nor gelatinase production was more common in disease-associated isolates than in isolates colonizing healthy individuals (ca. 60 to 65%). The majority of gelatinase-negative isolates, including 14 endocarditis isolates (of 80 isolates tested), contained the previo...

AIM: The aim is to compare the antimicrobial efficacy of calcium hydroxide, combination sesame oil with chitosan paste and polyethylene glycol intracanal medicaments on Enterococcus Faecalis at 72 hours, 8 days 14 respectively. Materials Methodology: A total single-rooted anterior teeth were selected, root canal preparation was done, divided into three groups contaminated E. faecalis, which fur...

The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing system. We recently developed a novel system for studying E. faecalis pathogenicity that involves killing of the nematode worm Caenorhabditis elegans and showed that an E. faecalis fsrB mutant (str...

The E test and the reference agar dilution methods were compared for detecting high-level aminoglycoside resistance (HLAR) among 71 selected clinical isolates, including 62 Enterococcus faecalis and 9 Enterococcus faecium isolates. High-level gentamicin resistance alone was found in 11% (5 E. faecalis and 3 E. faecium strains) and high-level streptomycin resistance was found in 42% (28 E. faeca...