Actinoplanes deccanensis ATCC 21983, the producer of antibiotics lipiarmycin A3 and A4, furnished also a related antibiotic designated lipiarmycin B, active against Gram-positive bacteria, including anaerobes, and against Neisseria. The structures of the two major components, B3 and B4, were elucidated from their physico-chemical properties, 1H and 13C NMR spectra and fast atom bombardment mass...

The high levels of very long chain fatty acids found in ram spermatozoa are located almost exclusively in one of two separable species of sphingomyelin. Mass spectral analysis, including fast atom bombardment of the purified sphingomyelin, has shown the fatty acids to have a carbon chain length of between 28 and 34, with between four and six double bonds, and to belong predominantly to the n-3 ...

Natural Products, Saponins, Molecular W eight Determ ination, Sugar Sequence, Purity Control The application of field desorption and fast atom bombardment mass spectrometry for molecular weight determination and structural elucidation o f spirostanol and furostanol saponins isolated from Paris Polyphylla Sm. (Liliaceae) is reported. The capacity and limitations o f these methods for sugar seque...

The aim of the present study was to compare polar lipids of yeast and mycelial forms of both Candida albicans and Candida dubliniensis. Cultures were harvested from Lee's medium after incubation at 37 degrees C for 48 h. Yeast and mycelial forms were washed, separated from one another, dried and lipids extracted and prepared for fast atom bombardment mass spectrometry analysis in the negative-i...

The chiral-discriminating abilities of new triazole-linked host compounds (6, 7) toward amino acid esters were evaluated by the FABMS/EL-guest method. Two types of host appending saccharide derivatives were synthesized by the copper-catalyzed Huisgen-1,3-dipolar cycloaddition (CuAAC). Host compounds binding constants and chiral-discriminating abilities toward the enantiomeric alanine esters wer...

@ Neutron and gamma radiation levels around a Cockcroft-Walton neutron generator producing 14-Mev neutrons at an approximate rate of 1010 neutrons per second were measured under conditions typical of those required for activation analysis. Neutron flux reduction to acceptable levels for limited operation was accomplished by 12 feet of distance that included shielding consisting of 27 inches of ...

Our approach to protein-protein docking includes three main steps. First, we run PIPER, a rigid body docking program based on the Fast Fourier Transform (FFT) correlation approach, extended to use pairwise interactions potentials. Second, the 1000 best energy conformations are clustered, and the 30 largest clusters are retained for refinement. Third, the stability of the clusters is analyzed by...

The H- and D-atom products from collisional quenching of OD A (2)Σ(+) by H(2) are characterized through Doppler spectroscopy using two-photon (2 (2)S ←← 1 (2)S) laser-induced fluorescence. Partial deuteration enables separation of the channel forming H + HOD products, which accounts for 75% of reactive quenching events, from the D + H(2)O product channel. The Doppler profiles, along with those ...

A new strategy for the structural characterisation of human albumin variants has been developed which makes extensive use of mass spectrometric methodologies. The rationale behind the method is to provide a rapid and effective screening of the entire albumin structure. The first step in this strategy consists in the attempt to determine the accurate molecular mass of the intact variant by elect...

Fast radio bursts (FRBs) are millisecond-duration events thought to originate beyond the Milky Way galaxy. Uncertainty surrounding the burst sources, and their propagation through intervening plasma, has limited their use as cosmological probes. We report on a mildly dispersed (dispersion measure 266.5 ± 0.1 parsecs per cubic centimeter), exceptionally intense (120 ± 30 janskys), linearly polar...