IT was demonstrated by Giardini and Nizetic (1949), using the Amsler-Huber fluorescein test (1949), that when guinea-pigs are made to breathe air containing approximately 5.52 to 4.16 per cent. oxygen (corresponding to the atmosphere at an altitude of 10-12,000 metres, or 35,000-70,000 feet) for one hour, there is an increase in the concentration of the fluorescein in the anterior chamber. When...

AIM This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. METHODS Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central c...

Failure to account for fluorescein absorbance changes with pH may be responsible for some of the apparent non-conservative behaviour of this easily detectable tracer compound. While it is possible to calculate an accurate absorptivity value for fluorescein at every pH, this calculation is not necessary if the sample pH is increased above pH 9 before measuring the absorbance. Intense sunlight de...

Fluorescein iris angiography and fluorophotometry were performed on a series of 9 patients with bilateral and 11 with unilateral pseudoexfoliation, 12 bilateral aphakes with pseudoexfoliation, and 7 unilateral aphakes with bilateral pseudoexfoliation. Angiography showed a loss of radial iris vessels, a heavy leak of fluorescein from the pupil margin, progressive neovascularisation of the outer ...

PURPOSE Laser-targeted angiography has unique advantages over conventional angiography of the fundus. Its efficacy in visualizing choroidal neovascular membranes was tested in a rat model and compared to that of fluorescein angiography. METHOD Laser-targeted angiography was performed in rats with choroidal neovascularization (CNV) by injecting heat-sensitive carboxyfluorescein liposomes intra...

Posterior cerebral artery (ACP) aneurysms account for less than 2% of all aneurysms. The fronto-temporo-zygomatic approach allows access to the cranial base with parenchymal damage. Endoscopy you visualize deep structures. Fluorescein verify permeability after clipping.

Lymphoid cells from 20 patients with lymphoproliferative disorders, including chronic lymphocytic leukemia, hairy cell leukemia, Sezary syndrome, lymphoma, and lymphadenitis, were studied for redistribution of surface membrane immunoglobulins (SmIg) and concanavalin A (Con-A) receptors. Fluorescein-labeled polyvalent goat anti-human immunoglobulin and fluoresceinated concanavalin A were used as...

Rhodamine-conjugated capsular polysaccharides isolated from Rhizobium japonicum 61A76NS were used to examine binding between the labeled polysaccharides and soybean roots. Fluorescein-labeled polysaccharides were not satisfactory because soybean root hairs autofluoresce in the fluorescein region.