This review analyses the literature concerning non-fluorescent and fluorescent probes for nucleic acid imaging in fixed and living cells from the point of view of their suitability for imaging intracellular native RNA and DNA. Attention is mainly paid to fluorescent probes for fluorescence microscopy imaging. Requirements for the target-binding part and the fluorophore making up the probe are f...

In this paper a forward solver software for the time domain and the CW domain based on the Born approximation for simulating the effect of small localized fluorophores embedded in a non-fluorescent biological tissue is proposed. The fluorescence emission is treated with a mathematical model that describes the migration of photons from the source to the fluorophore and of emitted fluorescent pho...

With the combination of climate change and human encroachment on the territory of endangered species, information about what and where animals eat and how this is changing over time can help guide conservation efforts. Thure Cerling et al. compiled a 6-year record of nitrogen, carbon, oxygen, and hydrogen in the diet of a family of 4 Kenyan elephants by analyzing the isotopic content of the ele...

Placing an imaging spectrograph or related components capable of generating a spectrum between a microscope and the image intensifier of a conventional fluorescence lifetime imaging (FLIM) system creates a spectrally resolved FLIM (SFLIM). This arrangement provides a number of opportunities not readily available to conventional systems using bandpass filters. The examples include: simultaneous ...

For the first time we have applied a nanosecond fluorescence lifetime imaging (FLIM) system together with a hyperspectral fluorescence imaging device during a diagnostic investigation of wall paintings. The FLIM apparatus is based on a pulsed laser emitting nanosecond UV pulses at 337 nm for fluorescence excitation and on a nanosecond time-gated image intensifier for acquiring fluorescence emis...

PURPOSE Developed herein is a three-dimensional (3D) flow contrast imaging system leveraging advancements in the extension of laser speckle contrast imaging theories to deep tissues along with our recently developed finite-element diffuse correlation tomography (DCT) reconstruction scheme. This technique, termed speckle contrast diffuse correlation tomography (scDCT), enables incorporation of c...

Abstract Fluorescent probes are valuable tools to visualize non‐catalytic proteins in live cells. Currently, the majority of imaging reagents for based on “always‐on” fluorophores and use these usually necessitate a wash step remove unbounded before microscope imaging. Herein, we report arylamino‐substituted rhodamine as an activatable fluorophore protein We have shown induction arylamino struc...

A recently created tissue demonstrative technique, in light of label-free close infrared (NIR) polarimetric reflectance imaging for the characterization of histopathological tests of lung cancer cells, is displayed. The design, calibration, and testing of an automated NIR polarimetric imaging framework, with accentuation on lung cancer location, are advanced in detail. The depicted electro-opti...

Confocal reflectance microscopy and confocal Raman spectroscopy have shown potential for non-destructive analysis of samples at micron-scale resolutions. Current studies utilizing these techniques often employ large bench-top microscopes, and are not suited for use outside of laboratory settings. We have developed a microscope which combines laser scanning confocal reflectance imaging and confo...

The simultaneous capture of imaging data at multiple wavelengths across the electromagnetic spectrum is highly challenging, requiring complex and costly multispectral image devices. In this study, we investigate the feasibility of simultaneous multispectral imaging using conventional image sensors with color filter arrays via a novel comprehensive framework for numerical demultiplexing of the c...