introduction: in vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. despite using many types of in vitro media, an appropriate environment has not been reported yet. present study was designed to assess the effect of heated human follicular fluid (hhff), which is s...

UNLABELLED Our aim was to compare Corpus luteum (CL) development and blood plasma concentration of progesterone ([P4]) in thoroughbred mares after spontaneous ( CONTROL C) or human chorionic gonadotrophin (hCG)-induced ovulation. Lactating mares (C=12; hCG=21) were daily teased and mated during second oestrus post-partum. Treated mares received 2500 IU hCG i.v. at first day of behavioural oes...

BACKGROUND Prenatal testosterone (T) excess from days 30-90 of gestation disrupts gonadotropin surge and ovarian follicular dynamics and induces insulin resistance and functional hyperandrogenism in sheep. T treatment from days 60-90 of gestation produces a milder phenotype, albeit with reduced fecundity. Using this milder phenotype, the aim of this study was to understand the relative postnata...

BACKGROUND ADRB-2 was implicated in rodent ovarian functions, including initial follicular growth. In contrast, ADRB-2 expression and function in nonhuman primate and human ovary were not fully known but innervation and significant levels of norepinephrine (NE), which is a ligand at the ADRB-2, were reported in the ovary. METHODS We studied expression of ADRB-2 in human and rhesus monkey ovar...

This study was undertaken to determine whether fully grown oocytes isolated from the early antral follicles (2-8 mm size) of frozen-thawed buffalo ovaries are viable and can be rescued to undergo maturation, fertilization, and embryo development in vitro. Ovaries were cryopreserved just after being collected during slaughter from the local abattoir using in situ oocyte (ISO) cryopreservation. I...

Argyrophilic nucleolar organiser regions (AgNORs) in 28 follicular lymphomas and 30 lymph nodes showing reactive follicular hyperplasia were studied to see if they were helpful in distinguishing follicular lymphoma from follicular hyperplasia in paraffin wax tissue sections. Mean nuclear counts were greater in follicular hyperplasia (3.71 v 3.11). This difference was significant but counts over...