This study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of Cronobacter sakazakii in powdered infant formula (PIF). To determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of C. sakazakii (2 × 100 an...

This study evaluated the effects of changing the composition of the pre-enrichment medium buffered peptone water (BPW) on the growth of stressed and unstressed Gram-negative foodborne pathogens in a one-broth enrichment strategy. BPW supplemented with an available iron source and sodium pyruvate, along with low levels of 8-hydroxyquinoline and sodium deoxycholate (BPW-S) improved the recovery o...

Quantitative description of food webs provides fundamental information for the understanding of population, community, and ecosystem dynamics. Recently, stable isotope mixing models have been widely used to quantify dietary proportions of different food resources to a focal consumer. Here we propose a novel mixing model (IsoWeb) that estimates diet proportions of all consumers in a food web bas...

Consumer awareness of the health benefits of n-3 fatty acids is growing and is driving consumer demand for enriched food products. Enrichment of meat with n-3 fatty acids is an opportunity for the broiler production sector to add value to their product, but enrichment can increase the cost of production. A study was conducted to determine an optimal production strategy for n-3 enrichment of bro...

The utility of the U.S. Department of Agriculture/Agricultural Research Service (USDA/ARS) package rinse method for recovering Listeria monocytogenes from the surface of contaminated foods was validated in comparison to the standard USDA/Food Safety and Inspection Service (FSIS) product composite enrichment method and two other methods using frankfurters from a lot with a known package prevalen...

Sensory experience modifies behavior through both associative and non-associative learning. In Caenorhabditis elegans, pairing odor with food deprivation results in aversive olfactory learning, and pairing odor with food results in appetitive learning. Aversive learning requires nuclear translocation of the cGMP-dependent protein kinase EGL-4 in AWC olfactory neurons and an insulin signal from ...

CARLQUIST, P. R. 1956 A biochemical test for separating paracolon grouips. J. Bacteriol., 71, 339-341. HA.NA, A. A. 1945 Triple-sugar iron agar medium for the identification of the intestinal group of bacteria. J. Bacteriol., 49, 516-517. KAUFFMANN, F. 1951 Enterobacteriaceae. Ejnar Munksgaard Ltd., Copenhagen, Denmark. SILLIKER, J. H. AND TAYLOR, W. I. 1958 Isolation of salmonellae from food s...

Primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, were coupled with oligonucleotides for the shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. A minimum of 10(2) CFU per PCR (10 microliters) was necessary to amplify E. coli O157:H7-specific bands by multiplex PCR. Food particles as well as various unknown metabolic by-products of bacteria ...

We used a subsidy–stress model as a basis for predicting macroinvertebrate community response to a steep gradient of P enrichment in the Florida Everglades, a P-limited wetland ecosystem. We tested the hypothesis that consumers were resource limited and their biomass would show a subsidy response (increase) to low-to-moderate levels of P enrichment, but a stress response (decrease) at high leve...

Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were cultured, from which 157 Listeria isolates. (14.3%) grew. When compared with modified McBride a...