Food pathogens contaminate food products that allow their growth on the shelf and also under refrigerated conditions. Therefore, it is of utmost importance to lower the limit of detection (LOD) of the method used and to obtain the results within hours to few days. Biosensor methods exploit the available technologies to individuate and provide an approximate quantification of the bacteria presen...

The aim of current studies was to validate the direct plating of a stool sample for Listeria monocytogenes detection, using selective medium Palcam agar with Palcam selective supplement. Validation was performed using stool samples collected from healthy humans inoculated with Listeria sp. strains. Stool samples were frozen to determine the influence of freezing on method robustness. The presen...

The need for rapid methods in order to precisely detect methicillin-resistant Staphylococcus aureus (MRSA) is extensively acknowledged. This study evaluated a quantitative real-time PCR assay targeting mecA (encoding high level resistance to methicillin) and femB (a specific genomic marker for S. aureus) genes to detect MRSA from broth culture, from serum seeded with MRSA and straight from the ...

In a fatigue-limit model, units tested below the fatigue limit (also known as the threshold stress) theoretically will never fail. This article uses a random fatigue-limit model to describe (a) the dependence of fatigue life on the stress level, (b) the variation in fatigue life, and (c) the unit-to-unit variation in the fatigue limit. We t the model to actual fatigue data sets by maximum likel...

We first prove a generalized Brill-Noether theorem for linear series with prescribed multivanishing sequences on smooth curves. We then apply this theorem to prove that spaces of limit linear series have the expected dimension for a certain class of curves not of compact type, whenever the gluing conditions in the definition of limit linear series impose the maximal codimension. Finally, we inv...

Liénard systems of the form ẍ + ǫf(x)ẋ + x = 0, with f(x) an even continous function, are considered. The bifurcation curves of limit cycles are calculated exactly in the weak (ǫ → 0) and in the strongly (ǫ → ∞) nonlinear regime in some examples. The number of limit cycles does not increase when ǫ increases from zero to infinity in all the cases analyzed.

The forming limit is an important failure criterion for polycrystalline sheet metals when approving the process. Recent developments in strain measurement technology, e.g., digital image correlation (DIC), enable evolution to be captured continuously and accurately. This new technology would improve measurements if onset of necking detection method was developed accordingly. paper proposes a ba...

Abstract One of the commonly used metal forming processes is deep drawing, in which circular blanks are converted into a cup or shell with good surface finish. In this paper, the forming characteristics of Deep Draw Steel (DDS), a low-carbon steel with many engineering and automotive applications, is investigated by deep drawing. The forming limit diagram is a useful concept in favor of charact...

We determine spectral curves for the known spiky string solutions in AdS space in the limit of large angular momentum. We also construct generic multi-spike solutions in this limit and compute the corresponding spectral data. The resulting spectral curves precisely match those of the classical spin chain describing the dual operators in one-loop gauge theory. Our results confirm the map between...