conclusions due to phenolic content, methanolic extract of m. piperita has moderate antibacterial and antifungal properties against the aforementioned gram positive and gram negative bacteria and fungi strains. materials and methods the methanolic extract was prepared by standard procedure, and total phenol, total flavonoid, and total anthocyanin content were determined using a spectrophotomete...

The attine ants of South and Central America are ancient farmers, having evolved a symbiosis with a fungal food crop >50 million years ago. The most evolutionarily derived attines are the Atta and Acromyrmex leafcutter ants, which harvest fresh leaves to feed their fungus. Acromyrmex and many other attines vertically transmit a mutualistic strain of Pseudonocardia and use antifungal compounds m...

PURPOSE To establish a murine model of corneal candidiasis that permits molecular evaluation of fungal adherence and invasion. METHODS Corneas of immunocompetent, methylprednisolone-treated, and cyclophosphamide-treated adult NIH Swiss and BALB/c mice were topically mock inoculated or inoculated with 10-fold increasing amounts between 100 and 100 million colony-forming units (CFU) of Candida ...

INTRODUCTION We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants. METHODOLOGY One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua El...

The hyphal wall protein 1 (HWP1) gene of Candida albicans encodes for a fungal cell wall protein, required for hyphal development and yeast adhesion to epithelial cells; yet, its role in pathogenesis remains largely unknown. In the present study, we analyzed two C. albicans laboratory strains, the DAY286 (HWP1/HWP1) and the null mutant FJS24 (hwp1/hwp1) and six clinical isolates [3 harbouring t...

BACKGROUND Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstock for biofuel production due to its abundance and low value. However, very limited study has been performed on lipid production by culturing oleaginous fungi with lignocellulosi...

24 25 The argasid ticks O. erraticus and O. moubata are of great medical and veterinary 26 importance because they are vectors of the African swine fever virus and several species of 27 human relapsing fever borreliae. Biocontrol of these ticks using entomopathogenic fungi has not 28 been previously reported. We examined the pathogenicity to different developmental stages of 29 these two argasi...

EXAMINATION OF NONSTERILE Preparation of Test Strains PRODUCTS: MICROBIAL Use standardized stable suspensions of test strains or prepare as stated below. Seed-lot culture maintenance techENUMERATION TESTS niques (seed-lot systems) are used so that the viable microorganisms used for inoculation are not more than five passages removed from the original master seed-lot. Grow each of the bacterial ...

Fungal infections are a global problem imposing considerable disease burden. One of the unmet needs in addressing these infections is rapid, sensitive diagnostics. A promising molecular diagnostic approach is high-resolution melt analysis (HRM). However, there has been little effort in leveraging HRM data for automated, objective identification of fungal species. The purpose of these studies wa...

Filamentous fungi are an important cause of pulmonary and systemic morbidity and mortality, and also cause corneal blindness and visual impairment worldwide. Utilizing in vitro neutrophil killing assays and a model of fungal infection of the cornea, we demonstrated that Dectin-1 dependent IL-6 production regulates expression of iron chelators, heme and siderophore binding proteins and hepcidin ...