The effect of hypotonic medium (Distilled water: DW) and hypertonic saline (HS: 5% NaCl) compared to control normal saline (NS) was studied on gastric ulcer induced by aspirin, 6 h cold restraint stress, ethanol, and pylorus ligation in rats. DW did not afford any protection while HS showed significant ulcer protective effects in all gastric ulcer models studied. The cytoprotective effect of HS...

This research aimed to determine the potential protective effects ofPhaleria macrocarpafruit extracton rat gastric mucosal injury induced by ethanol andto clarify the roles of prostaglandinE2 (PGE2), transforming growth factor1 (TGF) and tumor necrosis factorTNF). Main methods: Seven groups of rats were orally pretreated with Tween20 as vehicle control group; Tween20 as ulcer group; 20 mg/kg of...

This study was designed to compare the effect of oral administration of PGE2 (0.5 mg/kg) and ranitidine in larger (100 mg/dose) or smaller (10 mg/dose) doses on aspirin-induced gastric microbleeding and DNA loss determined chemically in gastric washings in eight healthy subjects. Aspirin (0.5 g) given four times daily greatly increased the rate of gastric bleeding and DNA loss and pretreatment ...

Gastric chief cells secrete pepsinogen and are found at the base of gastric glands. Chief cells constitutively express the anti-apoptotic protein Bcl-xL, which may enable them to live longer than other gastric cells. We reveal that the cytokines released as part of the inflammatory response during Helicobacter pylori infection dose-dependently disrupt chief cell monolayer integrity and viabilit...

Abstract Background Gastric carcinoma (GC) is a fatal disease. Detection of new biomarkers that can be utilized in the early diagnosis GC pressing need. This present study assessed centrosomal protein-55 (CEP55)’ serpin family E member 1 (SERPINE1) and sphingomyelin phosphodiesterase 3 (SMPD3) genes proteins gastric adenocarcinoma with different tumor progression features. Thirty surgically res...

Hydrogen sulfide (H2S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluate the effect of H2S on mRNA expression of NBC1, TFF1 and TFF2 in rat gastric mucosa in response to ...

Prostaglandin E(2) (PGE(2)) has a strong protective effect on the gastric mucosa in vivo; however, the molecular mechanism of a direct cytoprotective effect of PGE(2) on gastric mucosal cells has yet to be elucidated. Although we reported previously that PGE(2) inhibited gastric irritant-induced apoptotic DNA fragmentation in primary cultures of guinea pig gastric mucosal cells, we show here th...

The effect of 16,16-dimethyl prostaglandin E2 (dmPGE2) on gastric surface epithelial cell (SEC) damage induced by vagal nerve stimulation (VS) in urethane-anesthetized rats was studied using scanning electron microscopy. VS (1.25-10 Hz, 0.2 mA, 2 msec, 10 min) resulted in a graded increase in the SEC damage, increased gastric contractions, increased gastric acid secretion, and a decrease in hea...

The beneficial effects by peroxisome proliferator-activated receptor-γ (PPAR-γ) on gastric injury induced by ischemia-reperfusion have been confirmed, however, the precise mechanism of its cytoprotection is not elucidated thoroughly. The aim of the present study was to determine the gastric localization of PPAR-γ expression in the rat gastric mucosa, and to clarify the mechanism of its cytoprot...

Acemetacin is the carboxymethyl ester of indomethacin and a pro-drug precursor compound, in other words, prodrug. The pharmacologically active metabolite acemetacin also indomethacin. exerts its potent non-steroidal anti-inflammatory effects body as It known that not only prodrug indomethacin, but an agent with gastric protective mechanisms, possibly involving reduction leukocyte adhesion, by e...