Gel filtration chromatography, ultra-filtration, and solid-phase extraction silica gel clean-up were evaluated for their ability to remove microcystins selectively from extracts of cyanobacteria Spirulina samples after using the reversed-phase octadecylsilyl ODS cartridge for subsequent analysis by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The reversed-phase ODS cart...

Isolated frog retinas were incubated in vitro with a 4-h pulse of [3H]leucine, then chased for 32 h with a nonradioactive amino acid mixture. At the end of the incubation, light and electron microscope autoradiograms were prepared from some of the retinas. The autoradiograms revealed: (a) intense radioactivity in the basal disks of the rod outer segments, (b) diffuse label evenly distributed th...

p-Nitrophenol conjugating activity associated with liver microsomal UDP-glucuronosyltransferase (EC 2.4.1.17) was purified 150- to 200-fold from cell-free homogenates. The purification scheme included solubilization with the nonionic detergent Lubrol WX, anion exchange chromatography at pH 6.0 and 7.5, and affinity chromatography with UDP-hexanolamine Sepharose 4B. The enzyme purified as a phos...

The paper reports an efficient procedure for the extraction and purification of R-phycoerythrin (R-PE) from marine red algae Amphiroa anceps. A. anceps was extracted in 50 mM phosphate buffered saline and precipitated by (NH4)2SO4. The R-PE was isolated by ion-exchange chromatography loaded with Q-Sepharose Fast Flow, which was developed by linear ionic strength gradients. Then it was purified ...

In this study, we report the purification, initial structural characterization, and functional analysis of the molecular chaperone ClpB from the gram-positive, halophilic lactic acid bacterium Tetragenococcus halophilus. A recombinant T. halophilus ClpB (ClpB(Tha)) was overexpressed in Escherichia coli and purified by affinity chromatography, hydroxyapatite chromatography, and gel filtration ch...

We have developed a simple and inexpensive experiment using gel filtration chromatography that can be performed in less than 90 minutes. This procedure not only provides students with hands-on experience in both the preparation and use of chromatography columns, but also uses colored molecules to provide dramatic visual confirmation of the process of size-exclusion chromatography. Additionally,...

Two enzyme forms of alkaline phosphatase have been partially purified from the medium spent for the culture of HUH-6 clone 5 cells, which were originally derived from hepatoblastoma tissue. The purification methods used are ammonium sulfate precipitation, ethanol precipitation, diethylaminoethyl cellulose chromatography, Affi-Gel Blue chromatography, and Sephadex G-200 gel filtration. These alk...

A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitored by immunoprecipitation techniques. The recovery was found to be 35%, and the...

Bauhunia semibifida is among the several plants that have been studied for their medicinal properties. native to India. However, some species found in Africa. It has important uses, such as anti-bacterial, anti-inflammatory and anti-cancerous. This study aimed primarily at carrying out a preliminary phytochemical screening ascertain classes of bioactive compounds present solvent system(s) separ...

A zinc protein has been isolated and purified to apparent homogeneity from subjects with normal taste acuity by gel filtration and ion-exchange chromatography. The protein has a molecular weight of 37,000 and does not appear to have subunits. It is composed of 8% histidine residues and has 2 moles of zinc per mole of protein.