IN this issue of Genetics, Futschik and Schlötterer (2010) present one of the first and the most systematic explorations of the relative merits of pooling vs. individual sequencing for several genetics applications. They argue that pooling individuals is often more effective both for SNP discovery and for the estimation of allele frequencies (and thus for population genomic analyses) and as a r...

BACKGROUND Maize (Zea mays) is a C4 tropical cereal and its adaptation to temperate climates can be problematic due to low soil temperatures at early stages of establishment. METHODS In the current study we have firstly investigated the physiological response of twelve maize varieties, from a chilling condition adapted gene pool, to sub-optimal growth temperature during seedling emergence. To...

Culture-dependent and culture-independent approaches were used to determine the relationship between the dehalogenase gene pool in bacteria enriched and isolated on 2,2-dichloropropionic acid (22DCPA) and the environmental metagene pool (the collective gene pool of both the culturable and uncultured microbes) from which they were isolated. The dehalogenases in the pure-cultures isolates, which ...

MOTIVATION Because of the high cost of sequencing, the bulk of gene discovery is performed using anonymous cDNA microarrays. Though the clones on such arrays are easier and cheaper to construct and utilize than unigene and oligonucleotide arrays, they are there in proportion to their corresponding gene expression activity in the tissue being examined. The associated redundancy will be there in ...

The influence of phytochrome (high irradiance reaction; operationally, continuous far red light) on the incorporation of [(3)H]uridine into the cytoplasmic 2.5 megadalton precursor rRNA in the cotyledons of mustard (Sinapis alba L.) seedlings has been investigated. After irradiating 36-hour-old etiolated seedlings with 12 hours of far red light, the rate of incorporation is stimulated about 2-f...

Defects in DNA replication are associated with genetic instability and cancer development, as illustrated in Bloom syndrome. Features of this syndrome include a slowdown in replication speed, defective fork reactivation and high rates of sister chromatid exchange, with a general predisposition to cancer. Bloom syndrome is caused by mutations in the BLM gene encoding a RecQ helicase. Here we rep...

This research aimed to determine the variability of part sessile oak gene pool from area Natural Monument ?Kosutnjak Forest? at level one- and two-year-old seedlings, in progeny test established nursery Faculty Forestry. The was conducted with one-year-old seedlings 10 half-sib lines oak. heights root collar diameters were measured, survival end second growth period, as well annual height diame...

The development of health care and the pharmaceutical industry is based on principles Constitution Ukraine, fundamental European Union, basics medical law. Ministry Health Ukraine National Service are responsible for protection human rights freedoms, life health, patients, nation’s gene pool, as well privileged categories citizens. State institutions basis Laws regulations Resolutions Cabinet M...