In Iraq, Awassi is the most widespread sheep breed on non-European origin. The adapt to a wide range of environmental conditions. Sheep farming, mainly breed, represents an essential economic activity Iraq; however, so far, only very few genetic data exist this breed. present study was performed in Biology Department, University Zakho. Thirty two Blood samples were obtained from Awassi-Iq (Ovis...

PCR has become one of the most popular techniques in functional genomics. Projects in both forward and reverse genetics routinely require PCR amplification of thousands of samples. Processing samples to extract DNA of sufficient purity for PCR is often a limiting step. We have developed a simple 96-well plate-based high-throughput DNA extraction method that is applicable to many plant species. ...

Citation: Neelam M Nathani. “DNA Extraction Methods for Microbial Diversity Assessment from Marine Environments: Current Scenario and Future Perspectives”. EC Microbiology ECO.01 (2017): 55-56. COLUMN ARTICLE Neelam M Nathani Maharaja Krishnakumarsinhji Bhavnagar University India “Microbial diversity analysis from marine niche is adding huge number of novel microbial genomic sequences reconstru...

We have developed a novel nuclei extraction method that allows for the extraction of high molecular weight DNA from leaves of woody perennial soft-fruit species that contain high levels of carbohydrates and polyphenolics. The method utilizes a modified buffer system including 4% (w/v) polyvinylpyrrolidone (PVP)-10 and a combination of nylon filters and Percoll gradients to purify nuclei extract...

Background: The prevailing methods for the genomic DNA extraction of Sclerotium rolfsii from its mycelium mat is often time consuming and yields poor quality quantity owing to presence outrageous magnitude mucilage polysaccharides. Methods: A new fast track method isolation resting structure (sclerotia) S. by modifying CTAB deprived supplementation proteinase K has been standardized. Result: pr...

Human impact on marine benthic communities has traditionally been assessed using visible morphological traits and has focused on the macrobenthos, whereas the ecologically important organisms of the meio- and microbenthos have received less attention. DNA metabarcoding offers an alternative to this approach and enables a larger fraction of the biodiversity in marine sediments to be monitored in...

Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...

This study aims to evaluate the mutation status of KRAS, BRAF, NRAS, PIK3CA, and AKT analyze association gene mutations with clinicopathological characteristics colorectal cancer. 151 cancer patients were diagnosed at Pathology Molecular Biology Center in National Cancer Hospital K. The research was conducted as a cross-sectional using methods such genomic DNA extraction; analysis by Real-time ...

Recently, two cancer genomic profiling tests have been approved in Japan and implemented routine clinical practice: the FDA-approved FoundationOne CDx test, OncoGuide NCC Oncopanel test. The quality quantity of DNA significantly affects sequencing results; therefore, preparing a sufficient amount high-quality for is important. We examined best practices extraction from formalin-fixed paraffin-e...