Giardiasis is one of the most frequent entero-parasites worldwide; its prevalence is more common in developing countries. Giardia lamblia is considered one of the opportunistic parasites and its clinical manifestations represent the expression of host resistance and parasite virulence. As of chronic giardiasis cases were expected to worsen in immunosuppressed patients with development of variou...

Two new devices (OT, ST), were recently introduced for the recovery of parasite eggs and cysts for microscopic examination. These devices, two stationary flotation devices, and a standard double-centrifugal sugar-flotation were compared using common flotation solutions and methods recommended by the manufacturers for the recovery of hookworm, ascaridoid, and whipworm eggs from companion animal ...

Giardia and Cryptosporidium are important causes of diarrhoea in Bangladesh. The high prevalence of both parasites in humans and cattle in rural Bangladesh and the common use of water ponds by village inhabitants and their animals suggest a potential for zoonotic transmission. Direct transmission of Giardia and Cryptosporidium between cattle and their handlers and indirect transmission through ...

introduction: detection of cryptosporidium and giardia parasites in water samples is usually performed by us environmental protection agency 1623 method. nevertheless, the usepa1623 method still need improvement, to prevent and control the water borne parasitic disease. therefore, we undertook the present study. materials and methods: totally 48 surface water samples were collected. four sample...

This study evaluated the effect of peroxidation assisted by ultraviolet radiation (H2O2/UV), which is an advanced oxidation process (AOP), on Giardia duodenalis cysts. The cysts were inoculated in synthetic and surface water using a concentration of 12 g H2O2 L(-1) and a UV dose (λ = 254 nm) of 5,480 mJcm(-2). The aqueous solutions were concentrated using membrane filtration, and the organisms ...

Fecal specimens were collected from 30 calves from birth to 24 months of age at a dairy farm in Maryland to determine the prevalence of Giardia duodenalis genotypes in cattle of different ages. Fecal samples were subjected to density gradient centrifugation to remove debris and concentrate cysts. Specimens were analyzed by immunofluorescence microscopy and polymerase chain reaction (PCR). All P...

This study compared the recovery of Cryptosporidium oocysts and Giardia cysts ((oo)cysts) from raw waters using 4 different concentration-elution methods: flatbed membranes, FiltaMax foam, Envirochek HV capsules, and Hemoflow ultrafilters. The recovery efficiency of the combined immunomagnetic separation and staining steps was also determined. Analysis of variance of arcsine-transformed data de...

Giardia is a highly prevalent, understudied protistan parasite causing significant diarrheal disease worldwide. Its life cycle consists of two stages: infectious cysts ingested from contaminated food or water sources, and motile trophozoites that colonize and attach to the gut epithelium, later encysting to form new cysts that are excreted into the environment. Current understanding of parasite...