We have further explored the immunocytochemical staining method to discriminate renal and nonrenal hematuria, reported by Abrass and Laird (Am J Kidney Dis 1987;9: 44-50). After fixation on slides with acetone, erythrocytes in urine were stained with antiserum against human Tamm-Horsfall protein. Reactions were made visible by using either a fluorescent second antibody or a biotinylated second ...

Fish hematology is one of the most recent branches of physiology to assess the status of general health. Blood is the transport medium, a defense system, and an acid/base buffer system. Circulating blood is the common denominator of health and illness and alteration in its chemical or cellular illness and composition can indicate hematological and nonhematological diseases. Erythrocytes occupy ...

AIMS To analyse the cell cycle and DNA histogram components in data from DNA static cytometry and, in particular, to investigate the influence of the length of time the slides are exposed to the light of the cytophotometer in evaluating the G0/G1 peak. METHODS DNA static cytometry was performed on 18 Feulgen stained imprints and six histological sections taken from six breast carcinomas. The ...

BACKGROUND Histomorphological grading of cervical intraepithelial neoplasia (CIN) is crucial for clinical management. CIN grading is however subjective and affected by substantial rates of discordance among pathologists, which may lead to overtreatment. To minimise this problem, a histology review of CIN lesions by a consensus panel of pathologists is often used. Diffuse strong p16(INK4a) immun...

Background/Aim. Type IV collagen belongs to the group of nonfibrillar collagens and is an important component basement membranes, where it accounts for approximately 50% its structural elements. The aim study was describe expression distribution type in embryonic fetal metanephric kidney determine volume density tissue each trimester development. Methods. material consisted 19 human embryos/fet...

Malaria microscopy, microscopic examination of stained blood slides to detect parasite Plasmodium, is considered be a gold standard for detecting life-threatening disease malaria. Detecting the plasmodium requires skilled examiner and may take up 10 15 minutes completely go through whole slide. Due lack medical professionals in underdeveloped or resource-deficient regions, many cases misdiagnos...

The purpose of this pilot study was to determine the impact of the length of fixation in 10% buffered formalin on the expression of estrogen receptor by immunohistochemical analysis. We studied tissue samples from 10 invasive breast cancer cases after fixation for 1, 3, 6, and 9 to 10 hours. The tissue was processed immediately after fixation, resembling routine practice. Then the 40 blocks wer...

Malaria is an epidemic health disease and a rapid, accurate diagnosis is necessary for proper intervention. Generally, pathologists visually examine blood stained slides for malaria diagnosis. Nevertheless, this kind of visual inspection is subjective, error-prone and time-consuming. In order to overcome the issues, numerous methods of automatic malaria diagnosis have been proposed so far. In p...

Reticulocytes are precursory to the erythrocyte cells in the blood. They are anucleated cells, more rounded in shape and 20% greater, in volume, than the erythrocytes. However, when stained with panoptic dyes (Romanowski) they produce polychromatic slides, due to the presence of mature red blood cells with hemoglobin, synthesized during maturation, and reticulocytes with ribonucleic acid residu...