Cells of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803 supplemented with micromolar concentrations of L-[(14)C]arginine took up, concentrated, and catabolized this amino acid. Metabolism of L-[(14)C]arginine generated a set of labeled amino acids that included argininosuccinate, citrulline, glutamate, glutamine, ornithine, and proline. Production of [(14)C]ornithine preceded ...

1. Clostridium pasteurianum was grown on a synthetic medium with the following carbon sources: (a) (14)C-labelled glucose, alone or with unlabelled aspartate or glutamate, or (b) unlabelled glucose plus (14)C-labelled aspartate, glutamate, threonine, serine or glycine. The incorporation of (14)C into the amino acids of the cell protein was examined. 2. In both series of experiments carbon from ...

We evaluated feather meal (FTH) and poultry by-product meal (PBM) as complementary protein sources for growing calves. In a replicated 84-d growth trial, individually fed steer calves (n = 120; 252 +/- 24 kg) were supplemented with urea or with graded levels of soybean meal (SBM), FTH, PBM, or 2/3 FTH:1/3 PBM (CP basis). Protein efficiency, calculated as gain above the urea control vs natural p...

Two experiments were conducted to investigate the relationships between amino acids and DE for pigs weighing 20 to 50 kg. In Exp. 1, there were three dietary lysine levels that were either adjusted (1.50, 2.35 and 3.20 g/Mcal DE) for five DE levels (3.00 to 4.00 Mcal/kg) or unadjusted (.45, .71 and .96% of the diet) for three DE levels (3.50 to 4.00 Mcal/kg). In Exp. 2, diets containing six lys...

Rats given a lethal dose (LD(99.9)) of ammonium acetate (10.8 mmol/kg of body weight) were protected to the extent of 85 and 76% when previously injected with N-carbamoyl glutamate or L-arginine, respectively, at a level of 4 mmol/kg of body weight. At a dose of 1 mmol/kg of body weight, L-arginine protected 24%, while N-carbamoyl-L-glutamate protected 61% of the animals. When a combination of ...

We have isolated two Ca2+-binding proteins from squid optic lobes, each of which is also able to bind phenothiazines in a Ca2+-dependent manner. These proteins have each been purified and partly characterized. One of the proteins corresponds to calmodulin, in that it has a similar amino acid content to bovine brain calmodulin, including a single residue of trimethyl-lysine, it co-migrates with ...

lysine is nutritionally essential for humans and animals. it cannot be synthesized biologically in body. children and growing animals have a high requirement of lysine, since it is needed for bone formation. therefore, it must be added to food and feed supplement to improve the protein quality. in addition, lysine has some pharmaceutical applications in the formulation of diets with balanced am...

The response of glutamine and glutamate kinetics were studied in five healthy young adult men on diets containing deficient (0.1), adequate (0.8), or surfeit (2.2 g.kg-1.d-1) amounts of protein. Glutamate, glutamine, and phenylalanine kinetics were measured in the postabsorptive state at the end of each diet period. Urinary urea and ammonia excretion correlated with protein intake (the sum of t...

YAJIMA, Y., HIRASAWA, T. and SAHEKI, T. Treatment of Adult-Type Citrullinemia with Administration of Citrate. Tohoku J. exp. Med., 1981;, 134 (3), 321-330 A 48-year-old man who showed a regular diurnal fluctuation of blood ammonia level was diagnosed as adult-type citrullinemia with quantitative estimation of plasma amino acids and urea cycle enzymes in the liver. The restriction of daily prote...