The relative antithrombotic effectiveness of targeting glycoprotein (GP) Ib-dependent versus GPIIb-IIIa-dependent platelet interactions has been determined in baboons by measuring thrombus formation after infusing comparable antihemostatic doses of anti-von Willebrand factor (vWF) monoclonal antibody (MoAb) BB3-BD5, anti-GPIb MoAb AP1, and anti-GPIIb-IIIa MoAb LJ-CP8 under conditions of arteria...

Serum IgG or, predominantly, IgM antibody binding to electrophoretically separated normal platelet membrane protein antigens were detected by immunoblotting in five children with thrombocytopenia associated with varicella. Glycoproteins GPIb, GPIIb, GPIIIa, and other 25-260 kilodalton (kDa) proteins were identified as target antigens, suggesting a transient autoimmune mechanism causing the thro...

BACKGROUND Bernard-Soulier syndrome is a severe bleeding disease due to a defect of GPIb/IX/V, a platelet complex that binds the von Willebrand factor. Due to the rarity of the disease, there are reports only on a few cases compromising any attempt to establish correlations between genotype and phenotype. In order to identify any associations, we describe the largest case series ever reported, ...

A potent platelet glycoprotein Ib (GPIb) antagonist, crotalin, with a molecular weight of 30 kD was purified from the snake venom of Crotalus atrox. Crotalin specifically and dose dependently inhibited aggregation of human washed platelets induced by ristocetin with IC50 of 2.4 microg/mL (83 nmol/L). It was also active in inhibiting ristocetin-induced platelet aggregation of platelet-rich plasm...

An appealing strategy for developing therapeutic agents is to modify the design of human proteins, thereby taking advantage of target-specificity, multifunctionality of many proteins, and human compatibility—that is, provided any undesirable functional effects of the parent molecule can be selectively engineered away. In 2000, Cantwell & Di Cera1 reported a mutant form of the human serine prote...

Although it has been reported that activated platelets can adhere to intact endothelium, the receptors involved have not been fully characterized. Also, it is not clear whether activated platelets bind primarily to matrix proteins at sites of endothelial cell denudation or directly to endothelial cells. Thus, this study was designed to further clarify the mechanisms of activated platelet adhesi...

Shear-induced platelet aggregation (SIPA) involves von Willebrand Factor (vWF) binding to platelet glycoprotein (GP)Ib at high shear stress, followed by the activation of alphaIIb beta3. The purpose of this study was to determine the vWF sequences involved in SIPA by using monoclonal antibodies (MoAbs) to vWF known to interfere with its binding to GPIb and to alphaIIb beta3. Washed platelets we...

Mục tiêu: Đánh giá hoạt tính của yếu tố von Willebrand ở bệnh nhân di truyền tại Viện Huyết học Truyền máu Trung ương. Đối tượng và phương pháp nghiên cứu: Nghiên cứu mô tả cắt ngang trên 40 chẩn đoán (vWD) Kết quả: Nồng độ gắn GPIb (vWF: Act) collagen đều giảm đối cứu, cao nhất type 1 thấp 3. Tỷ lệ vWF CB/Ag Act/Ag 2 hơn so với 1, sự khác biệt có ý nghĩa thống kê p<0,001. 100% tỷ giảm, tron...