This study evaluates the growth and chemical composition of the following marine microalgae: Dunaliella tertiolecta, Isochrysis galbana, and Tetraselmis gracilis and the chemical composition of Chlorella pyrenoidosa. Microalgae can produce a number of compounds of high commercial value for the industry, mainly for the food industry. The growth kinetics, cell volume, pigments, carbohydrates, pro...

The large bloom-forming flagellate Gonyostomum semen has been hypothesized to be inedible to naturally occurring zooplankton due to its large cell size and ejection of long slimy threads (trichocysts) induced by physical stimulation. In a grazing experiment using radiolabelled algae and zooplankton collected from lakes with recurring blooms of G. semen and lakes that rarely experience blooms, w...

Genes encoding enzymes of the tetrapyrrole biosynthetic pathway were searched within Euglena gracilis EST databases and 454 genome reads and their 5' end regions were sequenced when not available. Phylogenetic analyses and protein localization predictions support the hypothesis concerning the presence of two separated tetrapyrrole pathways in E. gracilis. One of these pathways resembles the hem...

We evaluated the morphological framework of the pes anserinus in both knees of ten Nigerian cadavers and we observed high degree of variability in its morphology and location. The pes anserinus inserted specifically on the superior half of the media border of the tibia, as far inferiorly as 124.44 mm to the tibial tuberosity (prolonged insertion). The insertion was also joined to the part of ti...

Chorismate synthase was purified 1200-fold from Euglena gracilis. The molecular mass of the native enzyme is in the range of 110 to 138 kilodaltons as judged by gel filtration. The molecular mass of the subunit was determined to be 41.7 kilodaltons by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified chorismate synthase is associated with an NADPH-dependent flavin mononucleoti...

When Euglena gracilis was grown under white (fluorescent) light in media containing high concentrations of ammonium chloride (more than 0.005%), the main lipids synthesized were monogalactosyl diglyceride, digalactosyl diglyceride, phosphatidyl glycerol, sulfolipid and the all eis 47,10-16:2, 47,10,13-16:3, Zl4,7,10,13-16:4, A9,12-18:2 and A9,12,15-18:3 fatty acids. At low levels of ammonium (l...

Denis Dupuy1,10, Nicolas Bertin1,2,10, César A Hidalgo1,3,10, Kavitha Venkatesan1, Domena Tu4, David Lee4, Jennifer Rosenberg1, Nenad Svrzikapa1, Aurélie Blanc1, Alain Carnec1, Anne-Ruxandra Carvunis1, Rock Pulak5, Jane Shingles6, John Reece-Hoyes6, Rebecca Hunt-Newbury7, Ryan Viveiros7, William A Mohler8, Murat Tasan9, Frederick P Roth9, Christian Le Peuch2, Ian A Hope6, Robert Johnsen4, Donal...

Mercury contamination in aquatic systems poses a serious environmental stress to phototrophic plankton. We used Euglena gracilis gain an understanding of the physiochemical changes resulting from mercury across transcriptome and metabolome. Using combination Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) RNA-sequencing, we identified metabolomic transcriptomic both with...

Euglena gracilis (E. gracilis) accumulates paramylon, an immune-functional beta-glucan that can be used as a functional food. Paramylon production is strongly affected by the organic carbon source and initial pH conditions. Food processing byproducts have attracted attention for microalgal cultivation because of their low cost abundance nutrients, including nitrogen. We investigated optimal its...