In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

Small sized magnetite iron oxide nanoparticles (Fe3O4-NPs) with were successfully synthesized on the surface of rice straw using the quick precipitation method in the absence of any heat treatment. Ferric chloride (FeCl3·6H2O), ferrous chloride (FeCl2·4H2O), sodium hydroxide (NaOH) and urea (CH4N2O) were used as Fe3O4-NPs precursors, reducing agent and stabilizer, respectively. The rice straw f...

Resonance Raman spectra of ferric Aplysia myoglobin in the ligand-free and the azide-bound forms have been studied over a wide pH range to determine the coordination states of the heme iron atom. In the hydroxide form at high pH (-9) the iron is six-coordinate and is in a high/low spin equilibrium. As the pH is lowered below the acid/alkaline transition (pK, = 7.5), the heme becomes five-coordi...

The final step in the erythrocyte methemoglobin reduction pathway, the transfer of an electron from cytochrome b5 to methemoglobin, has been studied using magnetic circular dichroism spectroscopy. Spectral analysis allowed us to determine accurately the concentration of each redox species in mixtures of the two heme-proteins and to follow simultaneously the kinetics of the appearance or disappe...

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

High-valent iron-oxo species are thought to be intermediates in the catalytic cycles of oxygenases and peroxidases. An attractive route to these iron-oxo intermediates involves laser flash-quench oxidation of ferric hemes, as demonstrated by our work on the ferryl (compound II) and ferryl porphyrin radical cation (compound I) intermediates of horseradish peroxidase. Extension of this work to in...

Ferric chloride, when boiled with hydrazine hydrate and cacodylic acid, is converted into a fine cationic iron (ferric hydroxide) colloid which consists of 0.5-1.5 nm electron-dense granules, and gives a distinct Prussian blue reaction. This colloid allows light and electron microscopic detection of ionized anionic sites in tissues at a wide pH range of 0.8-7.6. It is smaller in size and more s...

Risperidone is a white to slightly beige powder. It is practically insoluble in water, freely soluble in methylene chloride, and soluble in methanol and 0.1 N HCl. RISPERDAL® tablets are available in 0.25 mg (dark yellow), 0.5 mg (red-brown), 1 mg (white), 2 mg (orange), 3 mg (yellow), and 4 mg (green) strengths. Inactive ingredients are colloidal silicon dioxide, hypromellose, lactose, magnesi...

Creating an artificial functional mimic of the mitochondrial enzyme cytochrome c oxidase (CcO) has been a long-term goal of the scientific community as such a mimic will not only add to our fundamental understanding of how CcO works but may also pave the way for efficient electrocatalysts for oxygen reduction in hydrogen/oxygen fuel cells. Here we develop an electrocatalyst for reducing oxygen ...