Interleukin (IL)-31 is mainly produced by CD4+ T cells, in particular T cells skewed toward a Th2 phenotype. Here we report for the first time that IL-31 stimulates secretion of proinflammatory cytokines, chemokines and matrix metalloproteinases (MMPs) from human colonic subepithelial myofibroblasts (SEMFs). The effects of IL-31 were investigated by cDNA microarrays, enzyme-linked immunosorbent...

Components of the Par-complex, atypical PKC and Par3, have been found to be downregulated upon activation of NF-κB in intestinal epithelial cells. To determine their possible role in pro-inflammatory responses we transduced Caco-2 human colon carcinoma cells with constitutively active (ca) PKCι or anti-Par3 shRNA-expressing lentiviral particles. Contrary to previous reports in other cell types,...

Enhancer of split (E(spl)), one of the neurogenic loci of Drosophila, is uncovered by the deletion Df(3R)E(spl)(R-B251) with breakpoints at 96F8 and 96F13. We describe here the results of a genetic analysis of this chromosomal interval. Thirty-one mutations in genes of this region were recovered during various programs of mutagenesis. In addition, we included the spontaneous mutations E(spl)(D)...

The Duffy Ag expressed on RBCs, capillaries, and postcapillary venular endothelial cells binds selective CXC and CC chemokines with high affinity. Cells transfected with the Duffy Ag internalize but do not degrade chemokine ligand. It has been proposed that Duffy Ag transports chemokines across the endothelium. We hypothesized that Duffy Ag participates in the movement of chemokines across the ...

The transcriptional repressor Hes1, a basic helix-loop-helix family protein, periodically changes its expression in the presomitic mesoderm. Its periodic pattern of expression is retained in a number of cultured murine cell lines. In this paper, we introduce an extended mathematical model for Hes1 oscillatory expression that includes regulation of Hes1 transcription by Drosophila Groucho (Gro) ...

This is the first of two papers in which we address the physics of the low-frequency Quasi-Periodic Oscillation (QPO) of X-ray binaries, in particular those hosting a black hole. We discuss and repeat the recent analysis and spectral modelling of the micro-quasar GRO J165540 by Sobczak et al. (2000, hereafter SMR), and compare it with GRS 1915+105; this leads us to confirm and analyze in more d...

The DNA-binding transcription factor Suppressor of Hairless [Su(H)] functions as an activator during Notch (N) pathway signaling, but can act as a repressor in the absence of signaling. Hairless (H), a novel Drosophila protein, binds to Su(H) and has been proposed to antagonize N signaling by inhibiting DNA binding by Su(H). Here we show that, in vitro, H directly binds two corepressor proteins...

Let G be a connected graph, where k2. S. Smith conjectured that every two longest cycles of G have at least k vertices in common. In this note, we show that every two longest cycles meet in at least ck 3Â5 vertices, where cr0.2615. 1998 Academic Press In this note, we provide a lower bound on the number of vertices in the intersection of any two longest cycles in a k-connected graph (k2). This ...

The genome-wide identification of microRNA transcription start sites (miRNA TSSs) is essential for understanding how miRNAs are regulated in development and disease. In this study, we developed mirSTP (mirna transcription Start sites Tracking Program), a probabilistic model for identifying active miRNA TSSs from nascent transcriptomes generated by global run-on sequencing (GRO-seq) and precisio...