Stem cells from the adult hair follicle bulge can differentiate into neurons and glia, which is advantageous for the development of an autologous cell-based therapy for neurological diseases. Consequently, bulge stem cells from plucked hair may increase opportunities for personalized neuroregenerative therapy. Hairs were plucked from the scalps of healthy donors, and the bulges were cultured wi...

In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by tran...

Normal hair growth occurs in cycles, comprising (anagen), cessation (catagen) and rest (telogen). Upon aging, the initiation of anagen is significantly delayed, which results impaired regeneration. Hair regeneration driven by follicle stem cells (HFSCs). We show here that aged HFSCs present with a decrease canonical Wnt signaling shift towards non-canonical Wnt5a antagonizes signaling. Elevated...

We report the presence of pluripotent neural crest stem cells in the adult mammalian hair follicle. Numerous neural crest cells reside in the outer root sheath from the bulge to the matrix at the base of the follicle. Bulge explants from adult mouse whisker follicles yield migratory neural crest cells, which in clonal culture form colonies consisting of over a thousand cells. Clones contain neu...

Hair follicle cyclical regeneration is regulated by epithelial-mesenchymal interactions. During androgenetic alopecia (AGA), hair stem cells (HFSC) differentiation impaired deregulation of dermal papilla (DPC) secreted factors. We analyzed androgen influence on BMPs expression in DPC and their effect HFSC to lineage. Androgens downregulated BMP2 BMP4 spheroids. Addition restored alkaline phosph...

Cellular hair growth therapy is an emerging approach to alopecia. Recently, Tsuboi et al. (J Am Acad Dermatol. 2020;83,109–116.) have observed hair-growth stimulation by dermal-sheath-cup cells in patients with androgenic Hair follicle-associated pluripotent (HAP) stem cells, originate the bulge area and express nestin, been shown differentiate multiple cell types including keratinocytes, neuro...

The cutaneous healing response has evolved to occur rapidly, in order to minimize infection and to re-establish epithelial homeostasis. Rapid healing is achieved through complex coordination of multiple cell types, which importantly includes specific cell populations within the hair follicle (HF). Under physiological conditions, the epithelial compartments of HF and interfollicular epidermis re...

The atypical protein kinase C (aPKC) is a key regulator of polarity and cell fate in lower organisms. However, whether mammalian aPKCs control stem cells and fate in vivo is not known. Here we show that loss of aPKCλ in a self-renewing epithelium, the epidermis, disturbed tissue homeostasis, differentiation, and stem cell dynamics, causing progressive changes in this tissue. This was accompanie...