Aim: Hepatitis B(HBV), C(HCV) and HIV infections are important parenterally transmitted infections. The aim of this study is to determine the seroprevalence HBsAg, anti-HBs, anti-HCV anti-HIV in patients with preoperative preparation.
 Materials MethodS: Anti-HBs, anti-HCV, tests chemiluminescent enzyme immune-assay method a total 900 applied General Surgery Clinic were evaluated preoperat...

Selection of antigens for therapeutic vaccination against chronic viral infections is complicated by pathogen genetic variations. We tested whether antigens present during persistent viral infections could provide a personalized antigenic reservoir for therapeutic T cell expansion in humans. We focused our study on the HBV surface antigen (HBsAg), which is present in microgram quantities in the...

Hepatitis B virus genotype E (HBV/E) is highly prevalent in Western Africa. In this work, 30 HBV/E isolates from HBsAg positive Angolans (staff and visitors of a private hospital in Luanda) were genetically characterized: 16 of them were completely sequenced and the pre-S/S sequences of the remaining 14 were determined. A high proportion (12/30, 40%) of subjects tested positive for both HBsAg a...

BACKGROUND Typically, DNA immunization via the intramuscular route induces specific, Th1-dominant immune responses. However, plasmids expressing viral proteins fused to cytotoxic T lymphocyte antigen 4 (CTLA-4) primed Th2-biased responses and were able to induced effective protection against viral challenge in the woodchuck model. Thus, we addressed the question in the mouse model how the Th1/T...

The study reports on the development of a novel hepatitis b vaccine prepared by physically mixing smaller microparticles (8-12 μm; blend PCL-PLGA; containing equivalent to 10μg HBsAg) with larger microparticles (12-20 μm; blend PCL-PLA; entrapping 10μg HBsAg). The vaccine was reconstituted with alum adsorbed HBsAg (equivalent to 10μg HBsAg) and was injected in BALB/c mice subcutaneously as sing...

Hepatitis B virus (HBV) is a DNA virus that infects the liver as primary target. Currently, a high affinity receptor for HBV is still unknown. The dendritic cell specific C-type lectin DC-SIGN is involved in pathogen recognition through mannose and fucose containing carbohydrates leading to the induction of an anti-viral immune response. Many glycosylated viruses subvert this immune surveillanc...

BACKGROUND Whether hepatitis B (HB) vaccine-conferred immunity persists into adulthood is unknown. We aimed to investigate long-term HB immunity in adolescents. METHODS In 2004-2005, 6156 high school students (15-21 years old) who had been vaccinated with plasma-derived HB vaccine as infants were recruited for HB seromarker screening. The immune response to an HB vaccine booster was evaluated...

Mutations in the human hepatitis B virus (HBV) genome contribute to its escape from host immune surveillance and result in persistent infections. The aim of this study was to characterize the molecular variations of the surface gene and protein in chronically-infected patients from the southern part of Iran. The surface genes from 12 HBV chronic carriers were amplified, sequenced and subsequent...

conclusions these findings indicated the association of the hbsag titer and serum ifnar2 in hbsag clearance in hepatitis b virus- infected patients. in consequence, immune mechanisms related to ifn-α/β signaling might be responsible in chb outcome. background loss of hepatitis b surface antigen (hbsag) in chronic hepatitis b (chb) infection is a favorable outcome. type i interferon (ifni) has a...