Correspondence to Andrew M. Fry: [email protected] A. Knebel’s present address is Medical Research Council Protein Phosphorylation Unit, The Sir James Black Center, Dundee DD1 5EH, Scotland, UK. Abbreviations used in this paper: HSP, heat shock protein; IF, immunofluorescence; IP, immunoprecipitate; KESTREL, kinase substrate–tracking and elucidation; LSB, low salt buffer; MT, microtubule; NEBD, nuc...

Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP). Currently, imidocarb dipropionate (ID) is only available drug for treating clinical form EP. Serious side effects incomplete clearance infection a major drawback ID. Heat-shock proteins (Hsp) play vital role in life cycle these haemoprotozoans by preventing alteration protein conformation. These Hsp activa...

After brief heat shock treatment, clinical strains of nontypeable Haemophilus influenzae show a long-lasting change in the binding specificity for glycolipids and a markedly increased growth rate in vitro. Non-heat-shocked H. influenzae specifically binds to phosphatidylethanolamine (PE), gangliotetraosylceramide (Gg4), and gangliotriosylceramide (Gg3) and binds minimally to sulfatoxygalactosyl...

the phenomenon of " hormesis ". Exposure of organisms to mild stress fortifies them against subsequent, more severe insults. Yet, the relevant molecular mechanisms remain poorly understood. Although heat stroke is often fatal and survivors may suffer permanent neurologic damage, the mechanisms underlying heat stroke-induced cell and tissue injury remain elusive. To gain insight, we employed the...

Folding of polypeptides in the cell typically requires the assistance of a set of proteins termed molecular chaperones. Chaperones are an essential group of proteins necessary for cell viability under both normal and stress conditions. There are several chaperone systems which carry out a multitude of functions all aimed towards insuring the proper folding of target proteins. Chaperones can ass...

Several mycobacterial antigens, identified by monoclonal antibodies and patient sera, have been found to be homologous to stress or heat-shock proteins (hsp) defined in Escherichia coli and yeast. A major antigen recognized by most Mycobacterium leprae-reactive human T cell lines and cell wall-reactive T cell clones is a 10-kD protein that has now been cloned and sequenced. The predicted amino ...

Wounding lettuce leaves induces the de novo synthesis of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), the accumulation of phenolic compounds, and subsequent tissue browning. A brief heat-shock at 45 C reduces the rise in wound-induced PAL, the accumulation of phenolic compounds, and tissue browning. The activity of PAL measured 24 h after wounding and the content of phenolic compounds (absorb...

∗ This minireview will be reprinted in the 2008 Minireview Compendium, which will be available in January, 2009. The authors’ work is currently supported by NIH Grants DA022354 and GM077430. 1 To whom correspondence should be addressed. Tel.: 734-936-5797; Fax: 734-763-4450; E-mail: [email protected] 2 The abbreviations used are: Hsp, heat shock protein; CHIP, carboxy terminus of Hsc70-interactin...

Organisms in natural habitats must frequently respond to changes in their environments through various physiological mechanisms. My laboratory has developed several methods for the quantification of stress in crustaceans. An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the American lobster (Homarus americanus). It is sensitive to as little as 0.2 fmol of peptide. Incr...

Heat shock proteins (Hsp) perform cytoprotective functions such as apoptosis regulation and inflammatory response control. These proteins can also be secreted to the extracellular medium, acting as inflammatory mediators, and their chaperone activity permits correct folding of proteins and avoids the aggregation of anomalous isoforms. Several studies have proposed the implication of Hsp in prio...