Background: Methylphenidate is one of the most common medications used for treatment of attention-deficit/hyperactivity disorder. It may be used for maintaining alertness and improving of attention which may lead to increase of the risk of substance abuse in some cases. The occurrence of alteration in male reproductive system such as hormonal secretion changes and alteration of testicular sperm...

Mechanisms of leukocyte adherence inhibition (LAI) phenomenon were investigated by utilizing Halliday's hemacytometer method. When guinea pig were immunized with dinitrophenyl conjugated bovine serum albumin (DNP BSA) with Freund's complete adjuvant, the adherence of their leukocytes to glass surface is markedly inhibited by the presence of BSA or PPD, but not by bovine gammaglobulin (BGG). Per...

Sperm chemoattraction in invertebrates can be sufficiently robust that one can place a pipette containing the attractive peptide into a sperm suspension and microscopically visualize sperm accumulation around the pipette. Sperm chemoattraction in vertebrates such as frogs, rodents and humans is more difficult to detect and requires quantitative assays. Such assays are of two major types - assay...

cell populations were freed of lymphocytes, so that the suppressive effect could be attributed specifically to macro phages. Brunner et a!. (5, 6) and Alexander et a!. (1) have shown that immune lymph node cells and lymphoid cells from the spleen can also eradicate tumor cells. This paper describes experiments in which allogeneic immune peritoneal cells are used for the eradication of cancer in...

Centrifugation is commonly used to wash sperm; however, most washing techniques do not put sperm in a chemically defined environment. Rather, washing by centrifugation, in effect, dilutes seminal plasma components. A 0.5-mL volume of 30% (wt/vol) Accudenz was layered beneath 5 mL of 12% (wt/vol) Accudenz in a 15-mL polypropylene centrifuge tube. Diluted semen from individual males (n = 10) was ...

Several papers have recorded the stimulation by 1,2,5,6-dibenzanthracene of the growth of a variety of organisms, such as Obelia (l) , Escherichia com-munior (2), planaria (3) , and Fusarium lini (4). The hydrocarbon has also been found to depress the respiration of brain, spleen, and liver (5). In view of the fact that cancer tissue is generally characterized by rapid growth and deficient resp...

Routine techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) include density centrifugation with Ficoll-Paque and isolation by cell preparation tubes (CPTs) and SepMate tubes with Lymphoprep. In a series of experiments, these three PBMC isolation techniques were compared for cell recovery and viability, PBMC population composition, and cell functionality, aiming to p...

BACKGROUND: In many animal models that investigate the pathology of various diseases, there is a need to monitor leukocyte counts and differentials. However, various researchers use a range of different techniques in male and female laboratory animals to collect such blood variable information. These studies are then compared to one another without consideration of the possibility that differen...