It is observed that some venom may have an anticancer effect. Hemiscorpius lepturus is a most dangerous scorpion in Iran. Heminecrolysin is the most toxic fraction of Hemiscorpius lepturus venom and it is responsible for hemolytic effects of the venom. In this research, we purified heminecrolysin by FPLC, and HPLC methods and used MTT assay in prostatic cancer cell line (PC3 cells). We observed...

An 88-year-old man with autoimmune hemolytic anemia (AIHA) who had been treated with low dose prednisolone developed a sudden worsening of his anemia accompanied by reactivation of Epstein-Barr virus (EBV). We established EBV-infected spontaneous lymphoblastoid cell lines (LCL), performed an enzyme-linked immunosorbent spot assay, and confirmed a significantly suppressed EBV-specific cytotoxic ...

From one strain of hemolytic streptococcus three forms were isolated, which produced three different degrees of hemolysis on the surface of blood agar in the presence of oxygen. The original form was moderately hemolytic; the glossy variant was more hemolytic than the original form; and the third form, obtained by passing the original culture through mice, was non-hemolytic. Under anaerobic con...

Isothermal titration calorimetry (ITC) is a useful tool for understanding the complete thermodynamic picture of a binding reaction. In biological sciences, macromolecular interactions are essential in understanding the machinery of the cell. Experimental conditions, such as buffer and temperature, can be tailored to the particular binding system being studied. However, careful planning is neede...

An isothermal titration calorimetric (ITC) method was developed to measure the kinetic parameters of ribonuclease A catalytic hydrolysis of cytidine 2',3'-cyclic monophosphate. Employing the inhibition of product as a probe, the K(m), K(i), k(c), and DeltaH(m) can be determined by two simple calorimetric measurements. First, the substrate was titrated into the cell containing high concentration...

Comparability between a biosimilar and its reference product requires the evaluation of critical quality attributes that may impact on its pharmacological response. Herein we present a physicochemical characterization of a biosimilar trastuzumab focused on the attributes related to the pharmacokinetic response. Capillary isoelectrofocusing (cIEF) and cation exchange chromatography (CEX) were us...

I t has often been noted that on a blood agar plate seeded with influenzal sputum the colonies of Bacillus influenz6e are most numerous and grow to larger size in the neighborhood of colonies of certain other organisms, notably the staphylococcus. This phenomenon led the authors to conduct various experiments which may be summarized as follows: Blood agar plates were seeded uniformly with a pur...

The structural significance of C-terminal amphiphilic alpha-helix of human interleukin-2 has been investigated using principles of protein design. Employing disulfide-mediated semi-synthesis, several multiple residue substitution patterns were studied in order to provide rapid insight into the most appropriate features to incorporate into fully recombinant proteins. Substitutions directed towar...

The measurement of total haemolytic complement is of diagnostic value in the rheumatic diseases. A method which is easier to perform than that of Kabat and Mayer (Experimental Immunochemistry, 2nd ed., Thomas, Springfield, Ill.) is described. A unit based on the mean value of the complement activities of normal sera is proposed to replace CH50 units. Other steps by which standardisation of the ...

Isothermal titration calorimetry (ITC) is commonly used to determine the thermodynamic parameters associated with the binding of a ligand to a host macromolecule. ITC has some advantages over common spectroscopic approaches for studying host/ligand interactions. For example, the heat released or absorbed when the two components interact is directly measured and does not require any exogenous re...