The objective of the study was to examine the activity of different biologically active proteins in geese egg whites. The experiment was performed in 8 replicates on eggs collected from Italian White geese, Polish wild geese (Gegawa) and their cross line. Analyses of activities of cystatin, lysozyme and serine proteinases inhibitors in fresh geese egg whites were carried out. Moreover, trypsin ...

An attempt to enhance the transglycosylation activity of lysozyme -'as made by chemical modification. Computer simulation of the Iysozyme-catalyzed reaction indicated that inhibition of the sugar residue binding te the binding subsite A caused a significant increase in transglycosylation activity. Therefore, the binary modification of Asp 1O1 and Trp 62 in hen egg white lysezyme was made in ord...

The concentration fluxes in the vicinity of protein crystals growing from its aqueous solution has been investigated using laser based interferometric technique. A Mach-Zehnder interferometer has employed for the experimentation. Lysozyme (Hen-Egg-White) has used as the protein, where sodium chloride (Nacl) is used as the precipitant and Sodium acetate (CH3COONa) as buffer. A test cell with dim...

We have investigated the effects of tyrosine nitration (to form the weak acid, 3-nitrotyrosine) at positions 23 or 20 plus 23, on the structure and function of hen egg-white lysozyme. Enzyme activity against Micrococcus luteus cell-wall fragments or soluble substrates exhibits two phenomena. (a) A decrease in Km and kcat for the hydrolysis of soluble oligo- and poly-saccharides, resulting in on...

The three-dimensional crystal structure of the complex between the Fab from the monoclonal anti-lysozyme antibody D1.3 and the antigen, hen egg white lysozyme, has been refined by crystallographic techniques using x-ray intensity data to 2.5-A resolution. The antibody contacts the antigen with residues from all its complementarity determining regions. Antigen residues 18-27 and 117-125 form a d...

In order to clarify the structural role of subsite B of hen egg-white lysozyme in hydrolytic activity towards a carbohydrate substrate, we analysed the structures of Trp-62-->Gly and Asp-101-->Gly mutant hen lysozymes, which have no side chain at positions 62 or 101, complexed with a substrate analogue, (N-acetyl-d-glucosamine)3 [(GlcNAc)3], using X-ray crystallography. The overall protein stru...

Divalent copper was found to inhibit non-competitively the lysis of Micrococcus lysodeikticus cells by hen egg-white lysozyme, with an inhibition constant K, = 3.8 X lo2 M-l. The association constants of Cu2+ for lysozyme and for a derivative of lysozyme in which tryptophan residue 108 was selectively modified, were measured spectrofluorimetrically and found to be 1.8 X lo2 M-l and 1.0 X lo3 M-...

This paper is presented to make both ocular symptoms and signs clear, by means of some experiments on the eye affected by photochemical air pollution. This paper summarizes three experiments, these results and discussion. The first series of experiments were performed in order to research eye irritation caused by photochemical synthetic irritants. They proved the threshold of eye irritation and...

References 1. Langeland TA. Clinical and immunological study of allergy to hen’s egg white VI. Occurrence of protein cross-reacting with allergens in hen’s white as studied in egg white from turkey, duck, goose, seagull and in hen egg yolk, and hen and chicken sera and flesh. Allergy 1983;38:399–412. 2. Takahashi K, Horiguchi M, Bando N, Tsuji H, Ogawa T, Asao T. Immunochemical characterization...