products were examined by Southern hybridization with internal oligonucleotide probes and by sequence analysis (not shown). In the study reported here, the ability of the reverse transcriptase enzyme to transcribe full-length cDNA from RNA was followed by a Long PCR reaction to amplify the entire enteroviral coding region together with the 5' and 3' nontranslated regions. The method was tested ...

The 17th–19th century wooden and stone churches are an iconic symbol for the Romanian national heritage, raising urgent needs development of efficient ecofriendly restoration preservation solutions. Nanotechnology has a great but largely unexplored potential in this field, providing new tools methods to achieve higher consolidation protection efficiency, mainly due ability nanoparticles inhibit...

Periodontitis is a chronic inflammatory disease characterized by the destruction of supporting structures teeth. Its high prevalence and negative effects on quality life make it one current problems in dentistry. Porphyromonas gingivalis (P. gingivalis) predominant periodontal pathogen that expresses number virulence factors involved pathogenesis periodontitis. P. fimbriae are critical factor i...

Background: In this study, the effects of three structural analogues of adenosine upon proliferation of human tumor cells were investigated. Previous research showed a cytotoxic effect of adenosine via A3 receptor and A1 receptor and sometimes this effect was receptor independent. The researches showed a differential cytotoxic effect of adenosine and its A3 agonists on cancerous cells, while ot...

Primary biliary cirrhosis (PBC) is an autoimmune liver disease characterized by the presence of autoantibodies to mitochondria (AMA). Recent evidence suggests that PBC develops after a locally driven response in the mucosa, where immunoglobulin A (IgA) is the dominant antibody isotype. In this study, we produced recombinant pyruvate dehydrogenase complex (PDC-E2)-specific dimeric human IgA mono...

disease, and a positive test result for antinuclear antibodies (ANAs) would not have been considered to have ssSSc. ANA positivity can occur in IPF patients, and thus we did not solely rely on this in our analysis. Rather, we highlighted the specificity of nucleolar staining for ANAs.3 In fact, in our previously described cohort of IPF subjects who were anti-Th/To-positive, we argued that, on r...

OBJECTIVES To report on a novel IIF pattern specifically associated with antibodies to DNA topo I. METHODS A novel compound IF pattern, designated Scl-70 pattern, was characterized in routine ANA-HEp-2 IIF screening. Within the last 3 years, all serum samples presenting the Scl-70 pattern at the ANA-HEp2 IIF screening were tested for anti-topo I reactivity. Conversely, 16 serum samples with k...

Three-dimensional (3D) cell cultivation is a powerful technique for monitoring and understanding diverse cellular mechanisms in developmental cancer and neuronal biology, tissue engineering, and drug development. 3D systems could relate better to in vivo models than two-dimensional (2D) cultures. Several factors, such as cell type, survival rate, proliferation rate, and gene and protein express...

Doxorubicin (Dox) is an anthracycline anticancer drug with high water solubility, whose use is limited primarily due to significant side effects. In this study it is shown that Dox interacts with monosialoglycosphingolipid (GM1) ganglioside micelles primarily through hydrophobic interactions independent of pH and ionic strength. In addition, Dox can be incorporated even into GM1 micelles alread...